Aseptic germination and rapid seedling method of liparis nervosa seeds

Abstract

The invention belongs to the technical field of plant culture, and particularly relates to an aseptic germination and rapid seedling method of liparis nervosa seeds. The method comprises the steps that serum capsules are soaked with ethyl alcohol and then sterilized, the sterilized serum capsules are cut apart and uniformly spread onto the surface of a liparis nervosa seed germination culture medium, after the serum capsules are germinated and grow leaves, inoculation is conducted for rooting culture, and rooted plants are subjected to cutting, proliferation and propagation expansion and thenacclimatized and transplanted. The liparis nervosa seed germination rate is high, the operation process is simple, and the rapid propagation period of liparis nervosa is shortened; the character expression of aseptic liparis nervosa seedlings is stabilized, and in the propagation process of the aseptic liparis nervosa seedlings, too many kinds of growth hormone can cause instability of the character expression. In the method, only two different kinds of culture medium formulas are adopted, the possibility of character variation is reduced, it is ensured that medicine components in the liparisnervosa tissue cultured seedlings are stable, and the quality of the medicine material is ensured from the source.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for aseptic germination and rapid seedling growth of sera seeds. Background technique [0002] See serum (Liparis nervosa) is a perennial terrestrial herb with 3 to 5 leaves in an adult plant, basal sheath-shaped clamshell, brown recumbent rhizomes developed, cylindrical pseudobulbs slender and slender, and racemes scattered , Capsule spindle-shaped. See the serum distributed in Sichuan, Guizhou, Chongqing and other places in the shady slopes of shady forests or sparse grasses, and it does not have a competitive advantage in dense forests and overgrown weeds and disappears. Traditionally, see serum is used as medicine with dried whole herb, and its most significant effect is to stop bleeding. Traditional Chinese medicine believes that see serum also has the effect of cooling blood, clearing away heat and detoxifying. Folks are often used for stoma...

AI Technical Summary

Problems solved by technology

Its purpose is: in view of the existing problems such as the difficulty in natural germination of serotonin seeds, the depletion of wild resources, and the endangered wild population, by screening the germination medium, effectively improve the germination rate of serotonin seeds; through the two links of aseptic germination and rooting culture Tight chimerism, compared with the conventional process, can effectively reduce the period of seedling seedling formation; through the use of serum seeds for aseptic germination and batch rapid seedling cultivation and seedling hardening technology, the rapid industrialization of seedlings can be effectively solved; for See serum wild resource population protection and industrial cultivation to provide technical support