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A Method for Proteome Detection by Pulsed Data-Independent Acquisition Mass Spectrometry

A mass spectrometry detection and proteome technology, which is applied in the field of pulsed data independent acquisition mass spectrometry detection proteome, can solve the problems of few protein species, low peptide resolution, insufficient resolution, etc., and achieves increased protein quantity, high quality, Reduce the effect of mutual influence

Active Publication Date: 2019-12-17
WESTLAKE OMICS (HANGZHOU) BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the deficiencies in the above-mentioned prior art, the present invention provides a method for detecting proteomes by pulsed data-independent acquisition mass spectrometry, and the present invention solves the problem that precursor ions formed by polypeptides cannot Due to insufficient separation and large mutual shadows, the resolution of the collected data for peptides is low, and there are few technical problems in the identification of protein types

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  • A Method for Proteome Detection by Pulsed Data-Independent Acquisition Mass Spectrometry
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  • A Method for Proteome Detection by Pulsed Data-Independent Acquisition Mass Spectrometry

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Embodiment 1

[0020] The specific technical scheme of the pulse-type data-independent acquisition mass spectrometry detection proteome method provided in this embodiment is as follows:

[0021] A method for detecting proteomes by pulsed data-independent acquisition mass spectrometry, comprising the following steps:

[0022] (1) Set 24 large windows, the interval width of the mass-to-charge ratio of the first 20 large windows is 20m / z, and the interval width of the mass-to-charge ratio of the last 4 large windows is 60m / z, 80m / z, 120m / z z and 140m / z;

[0023] (2) Divide the large window in step (1) evenly into 4 small windows to form 96 small windows, among which, the mass-to-charge ratio interval width of the first 80 small windows is 5m / z, and the last 16 small windows The interval width of the mass-to-charge ratio is 4 15m / z, 4 20m / z, 4 30m / z and 4 35m / z;

[0024] (3) Evenly distribute the 96 small windows in step (2) to 4 mass spectrometry acquisition methods for scanning; for each met...

Embodiment 2

[0028] The specific technical scheme of the pulse-type data-independent acquisition mass spectrometry detection proteome method provided in this embodiment is as follows:

[0029] A method for detecting proteomes by pulsed data-independent acquisition mass spectrometry, comprising the following steps:

[0030] (1) Set 24 large windows, the interval width of the mass-to-charge ratio of the first 20 large windows is 20m / z, and the interval width of the mass-to-charge ratio of the last 4 large windows is 60m / z, 80m / z, 120m / z z and 140m / z;

[0031] (2) Divide the large window in step (1) evenly into 4 small windows to form 96 small windows, of which the interval width of the mass-to-charge ratio of the first 80 small windows is 10 m / z, and the mass-to-charge ratio of the last 16 small windows is The interval width of charge ratio is 4 30m / z, 4 40m / z, 4 60m / z and 4 70m / z;

[0032] (3) Evenly distribute the 96 small windows in step (2) to 4 mass spectrometry acquisition methods fo...

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Abstract

A method for mass spectrometric detection of a proteome using pulsed data independent acquisition, comprising the following steps: (1) configuring M large windows, the interval width of the mass-to-charge ratio of the large windows being 2-150 m / z; (2) evenly dividing each large window into N small windows, the interval width of the mass-to-charge ratio of the small windows being 2-75 m / z; (3) evenly distributing the M*N small windows to N mass spectrometry acquisition methods for scanning, and for each acquisition method, selecting and fragmenting all ions in the M small windows, detecting all fragments generated by the irons in the windows, and obtaining acquisition data; and (4) merging the M*N pieces of acquisition data in the N mass spectrometry acquisition methods in step (3), and analyzing the obtained acquisition data. The method reduces mutual effects of precursor ions formed by polypeptides, so that the resolution of acquired data is improved, and the quantity of protein identifications is increased.

Description

technical field [0001] The invention belongs to the technical field of biological sample detection, and in particular relates to a method for proteome detection by pulsed data-independent acquisition mass spectrometry. Background technique [0002] Quantitative proteomics research can explain the causes and laws of the occurrence and development of certain biological phenomena from the protein level. , diabetes, etc.) is of great significance to the diagnosis and treatment. For example, by studying the differentially expressed proteome of tumor tissue and non-tumor tissue, it may be possible to discover a certain tumor-specifically expressed protein as a marker of disease, which can be used for early diagnosis, classification and prognosis of tumors. [0003] In proteomics research, complex samples such as human tissue often contain thousands of proteins, and current mass spectrometry techniques cannot detect all proteins simultaneously. At present, the most important anal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 郭天南朱怡蔡雪葛伟刚
Owner WESTLAKE OMICS (HANGZHOU) BIOTECHNOLOGY CO LTD
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