Liquid fertilizer for improving fruit-corn fruit quality and preparation method thereof
A liquid fertilizer, fruit corn technology, applied in the directions of liquid fertilizer, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of small fruit, less nutrients, poor taste, etc., and achieve easy absorption and nutritional balance. , to prevent the effect of empty fruit
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Embodiment 1
[0043] The isolation and identification of embodiment 1 Streptomyces bacterial strain (Streptomyces)
[0044] 1. Experimental materials and conditions
[0045] (1) Soil samples collected in a certain place in Chenzhou City, Hunan Province.
[0046](2) The components per liter of the liquid medium are as follows: 3.0 g of beef extract, 10.0 g of peptone, 5.0 g of sodium chloride, dilute to 1 liter of water, and the pH is 7.5.
[0047] The components per liter of solid medium are as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, agar 15-25g, water to 1 liter, pH 7.5.
[0048] The culture medium was divided into aliquots and sterilized in an autoclave (121°C, 30min).
[0049] (3) Sterile operating conditions: All utensils and utensils must be sterilized in an autoclave (121°C, 30min), and operations such as inoculation are carried out in an ultra-clean workbench.
[0050] (4) Conditions for culturing strains: place them in a light incubator (14L: 10D) at 25±1...
Embodiment 2
[0065] Embodiment 2 A kind of preparation of the liquid fertilizer that improves fruit corn fruit quality
[0066] A preparation method for improving the quality of fruit corn fruit comprises the following steps:
[0067] S1. Preparation of culture medium: soak 1000g of soybeans for 10 hours, put them into a refiner and grind them into soybean milk, filter out the bean dregs, put the filtrate in a clean fermenter, add white sugar to the filtrate, add water to The concentration of sugar in the medium is 100g / L, and the pH is 7.5;
[0068] S2. inoculation: get the liquid spawn of Streptomyces bacterial strain " TY-001# ", inoculate in the culture medium, stir evenly; There are 200,000,000 viable bacteria per milliliter in the liquid spawn; The ratio of liquid spawn and culture medium 165g:250L;
[0069] S3. Fermentation: Turn on the oxygen pump, continuously feed oxygen into the fermenter during the fermentation process, and add an appropriate amount of defoamer according to t...
Embodiment 3
[0072] Embodiment 3 A kind of preparation of the liquid fertilizer that improves fruit corn fruit quality
[0073] A preparation method for improving the quality of fruit corn fruit comprises the following steps:
[0074] S1. Preparation of culture medium: soak 1000g of soybeans for 10 hours, put them into a refiner and grind them into soybean milk, filter out the bean dregs, put the filtrate in a clean fermenter, add white sugar to the filtrate, add water to The concentration of sugar in the medium is 100g / L, and the pH is 7.5;
[0075] S2. inoculation: get the liquid spawn of Streptomyces bacterial strain " TY-001# ", inoculate in the culture medium, stir evenly; There are 200,000,000 viable bacteria per milliliter in the liquid spawn; The ratio of liquid spawn and culture medium 165g:250L;
[0076] S3. Fermentation: Turn on the oxygen pump, continuously feed oxygen into the fermenter during the fermentation process, and add an appropriate amount of defoamer according to t...
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