Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fermentation enzyme production method for Bacillus cereus producing cholesterol oxidase

A cholesterol oxidase and Bacillus cereus technology, applied in the field of bioengineering, can solve the problems affecting the final enzyme production of strains, fermentation effects, etc., and achieve the effects of easy control, stable enzyme activity, and short fermentation period

Pending Publication Date: 2019-07-02
DALIAN UNIVERSITY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Liquid fermentation technology is widely used in traditional industrial production due to its controllable fermentation conditions. However, during the fermentation process, many factors will affect the growth of microorganisms and the production of enzymes. The most important factor is the composition of the medium. Secondly, all other physical and chemical factors such as fermentation temperature, PH, metal ions, etc. will also affect the fermentation, and then affect the final enzyme production of the strain

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fermentation enzyme production method for Bacillus cereus producing cholesterol oxidase
  • Fermentation enzyme production method for Bacillus cereus producing cholesterol oxidase
  • Fermentation enzyme production method for Bacillus cereus producing cholesterol oxidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] The present invention is further described below in conjunction with specific embodiment, but not limited to following example. The experimental methods in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified; Bacillus cereus was purchased from China Industrial Microorganism Culture Collection and Management Center, and the preservation number is CICC20475.

[0019] A method for fermenting and producing cholesterol oxidase-producing Bacillus cereus, the method uses Bacillus cereus as the bacterial species to ferment and produce enzyme, and then prepare and produce cholesterol oxidase, and the specific steps are as follows:

[0020] (1) Preparation medium: yeast powder 5g, cholesterol 1g, Tween-80 1ml, NH 4 NO 3 1g, KH 2 PO 4 0.25g, MgSO 4 0.25g, FeSO 4 0.001g, 1L of water, pH 7.0, high temperature sterilization at ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a fermentation enzyme production method for Bacillus cereus producing cholesterol oxidase, which belongs to the field of bioengineering. In the method, a strain of the Bacillus cereus is used as a bacterial strain to perform fermentation enzyme production, thereby preparing the cholesterol oxidase. The fermentation medium and enzyme production method obtained by screeningand optimization has the advantages of short fermentation cycle, mild condition, easy control, provided optimal fermentation condition, high enzyme production efficiency, high enzyme activity, stableenzyme activity, and the like, and is beneficial to carry-out of application research in the next step.

Description

technical field [0001] The invention relates to a method for fermenting and producing cholesterol oxidase-producing Bacillus cereus, which belongs to the field of bioengineering. Background technique [0002] Cholesterol oxidase is a commercially valuable enzyme widely used for the determination of cholesterol in serum, other clinical samples, and food. In addition, the enzyme has potential applications as a biocatalyst that can be used as a pesticide and for the biotransformation of many sterols and non-sterols. How to quickly screen out sarcosine oxidase with high enzyme activity and high yield suitable for industrial production is of great significance, and the industrial production of sarcosine oxidase is mainly obtained by microbial fermentation. [0003] Liquid fermentation technology is widely used in traditional industrial production due to its controllable fermentation conditions. However, during the fermentation process, many factors will affect the growth of micr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N9/04C12R1/085
CPCC12N9/0006C12Y101/03006
Inventor 迟乃玉希伦张庆芳刘洋于爽
Owner DALIAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com