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Pesticide thiuram concentration detection test paper based on glutathione gold cluster and silver ion sensing system and its preparation method

A sensing system and detection test strip technology, applied in the field of biosensors, can solve the problems of cumbersome test methods, low detection sensitivity, and long test time, and achieve the effects of convenient observation, simple construction process, and low cost

Active Publication Date: 2021-03-30
JILIN UNIV
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method based on GSH-AuNCs and Ag to overcome the problems of long test time, large errors, cumbersome test methods and low detection sensitivity in the prior art. + Concentration detection test paper for the pesticide Thiram in the sensing system

Method used

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  • Pesticide thiuram concentration detection test paper based on glutathione gold cluster and silver ion sensing system and its preparation method
  • Pesticide thiuram concentration detection test paper based on glutathione gold cluster and silver ion sensing system and its preparation method
  • Pesticide thiuram concentration detection test paper based on glutathione gold cluster and silver ion sensing system and its preparation method

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Experimental program
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Embodiment 1

[0021] Example 1: Synthesis of GSH-AuNCs

[0022] In a water bath at 36°C, 20mmol L -1 concentration of HAuCl 4 The aqueous solution and deionized water are mixed according to the solution volume ratio of 1:9 and then stirred continuously. During the stirring process of the mixed solution, 100mmol L -1 The concentration of glutathione (GSH) aqueous solution, the volume ratio of GSH aqueous solution to the mixed solution is 1:27; after adding the GSH solution, continue to stir for 5 minutes, then heat the temperature to 70 ° C, continue to react for 24 hours, take out the mixed solution and put it in Dialyze in a dialysis bag (molecular weight cut-off 1 kDa) for 12 hours, take out the GSH-AuNCs solution in the dialysis bag and put it in a refrigerator at 4°C for use.

Embodiment 2

[0023] Embodiment 2: the making of test strip

[0024] Immerse the test paper (Hangzhou Fuyang Special Paper Co., Ltd., 0.6cm*0.6cm) into the solution containing GSH-AuNCs, 250 μmol L -1 AgNO 3 (GSH-AuNCs and AgNO 3 The solution volume ratio is 1:4, mixed in a 2mL centrifuge tube and left to stand for 5 minutes) in the mixed solution, immersed in the mixed solution for 12 hours, and then the test paper was taken out and dried to obtain the GSH-AuNCs-based / Ag + Thiram Concentration Test Strips from Sensing Systems. The color of the test paper is as figure 2 (a) shown.

Embodiment 3

[0025] Embodiment 3: the application of test paper to Thiram concentration detection

[0026] Based on GSH-AuNCs, Ag + The test paper of the system showed bright pinkish yellow under the ultraviolet light, and 100 μL of different concentrations of Thiram (2.5, 5, 7.5, 10, 15 μg mL) were added dropwise to the above test paper -1 ), the aggregation-enhanced fluorescence system on the test paper is quenched, and it can be observed that with the increase of Thiram concentration, the color of the test paper gradually changes from pink yellow to blue. The color change of the test paper is as follows: figure 2 (b) shown. The concentration of Thiram can be detected qualitatively by the color change. The histogram A of the relationship between the hue value and the Thiram concentration obtained after digital processing by the ImageJ software, and the linear diagram B of the relationship between the hue value and the Thiram concentration can quantitatively detect the Thiram concentra...

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Abstract

A pesticide thiram concentration test strip based on a glutathione gold cluster (GSH-AuNCs) and silver ion detection system and a preparation method thereof belong to the technical field of biosensors. The test strip is loaded with glutathione gold clusters and silver ions. According to the fluorescence aggregation enhancement effect of glutathione gold clusters and silver ions, the test paper impregnated with the mixture of gold clusters and silver ions shows bright pastel yellow under the ultraviolet light, and the fluorescence intensity will be quenched by thiram due to the specific bindingof thiram and silver ions after pesticide thiram solutions of different concentrations are added dropwise, so that fluorescence quenching of different degrees is produced and the test strip graduallyturns blue. The test strip created based on the above principle and provided by the invention can quickly detect thiram, has the advantages such as simple operation, high sensitivity and low cost, and provides a new method for instant and rapid test of pesticide concentration residue.

Description

technical field [0001] The invention belongs to the technical field of biosensors, in particular to a pesticide thiram concentration detection test paper based on a glutathione gold cluster (GSH-AuNCs) and silver ion sensing system and a preparation method thereof. Background technique [0002] Pesticides are very important to modern agriculture because they can effectively control the survival rate of pests that cause huge losses in crop production. According to previous data surveys, the use of pesticides can guarantee a one-third increase in global crop production. However, the abuse and improper treatment of pesticides make them infiltrate into soil, atmospheric circulation and water circulation, resulting in toxic pesticide residues in crops such as fruits and vegetables, further posing a serious threat to ecosystems and even human health. Therefore, it is an important guarantee for the development of human society to develop a convenient and reliable sensor for detect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 卢革宇赵旭闫旭郑杰刘方猛刘晓敏孙鹏高原梁喜双刘凤敏
Owner JILIN UNIV