Practical cell cryopreservation cooling box

Abstract

The invention discloses a practical cell cryopreservation cooling box which comprises a main body container A, a box cover, a sealing plug B, a sealing vacuum layer and a free cabin. The main body container A is of a cylindrical structure, the box cover is arranged at the upper part of the main body container A in a sleeved manner, and the sealing plug B is arranged in the box cover in a sleeved manner. The practical cell cryopreservation cooling box has the beneficial effects that the practical cell cryopreservation cooling box is extremely convenient and practical; as a 4 DEG C refrigerator,a -20 DEG C refrigerator and a -80 DEG C refrigerator are needed in conventional cell cryopreservation, the container is suspended in a liquid nitrogen tank directly, so that the demand on the threerefrigerators is cancelled and the step of transferring the container to different temperature environments is cancelled; the practical cell cryopreservation cooling box is large in capacity: in a same appearance volume, compared with a programmed cooling box circulating in the market, the cooling box can accommodate 40 2ml cell cryopreservation pipes at one time, far higher than 18, 2ml cell cryopreservation pipes accommodated in programmed cooling boxes in the market; the practical cell cryopreservation cooling box is high in cell survival rate: in cell cryopreservation in a state that the cell vitality is 100%, the survival cell proportion after revive can reach over 95% and is equal to or higher than that of the programmed cooling box in the market.

Description

technical field [0001] The invention relates to a cooling box, in particular to a practical cooling box for cryopreservation of cells, which belongs to the technical field of application of biomedical heat preservation instruments. Background technique [0002] In the use of the existing cell gradient freezing box, before freezing the cells, first take out the cryopreservation tube bracket in the box, add an organic solvent into the box, then put the bracket back into the box, and then pre-cool the whole box, When freezing, put the cryopreservation tube containing the cells into the cryopreservation box, screw the lid on, gradually lower the temperature from 4°C to -20°C, then drop to -80°C, then take out the cryopreservation box and open the lid , the cells were removed and stored in liquid nitrogen. [0003] The existing cell gradient cryopreservation box has the following disadvantages: when the bracket is put back into the box and the cover is unscrewed, the organic sol...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the organic solvent contained in the box is easy to leak out when the holder is put back into the box and the cover is unscrewed in the existing cell gradient freezing box, especially if the box is stored in- When the cells in the cryopreservation box at 80°C are taken out and moved to the liquid nitrogen tank, the lid of the cryopreservation box is difficult to unscrew because it is placed at sub-zero temperature. Solvent leakage problem, and proposed a practical cell cryopreservation cooling box

Images