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Antibacterial peptides and application thereof

An antibacterial peptide, a technology of any proportion, applied to the antibacterial peptide and its application field, can solve the problems of complex structure and high human cytotoxicity, and achieve the effects of low hemolytic activity, broad-spectrum antibacterial activity, and high antibacterial activity

Active Publication Date: 2019-08-02
QIQIHAR MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, antimicrobial peptides from natural sources also have disadvantages such as high toxicity to human cells and complex structures.

Method used

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  • Antibacterial peptides and application thereof
  • Antibacterial peptides and application thereof
  • Antibacterial peptides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation of embodiment 1 antimicrobial peptide

[0046] A polypeptide FC8 with antibacterial activity was designed by bioinformatics, and FC8 was modified from the number of charges and the overall hydrophobicity, and FC8-Q10K, FC8-Q7K / Q10K and FC8-Q7K / Q10K / Q21K, FC8-A12L, FC8-A5L / A12L, and FC8-A5L / A12L / A16L.

[0047] The above polypeptides were synthesized by solid phase synthesis.

[0048] After activation and deprotection of MBHA resin, according to the standard solid-phase peptide synthesis procedure, Fmoc-amino acid is used as raw material to synthesize sequentially from the C-terminus to the N-terminus of the polypeptide sequence. After the last amino acid has been added, acetylation is performed using acetic anhydride and the peptide is sheared and lyophilized. Use double-distilled water (phase A) containing 0.1% TFA and acetonitrile (phase B) containing 0.1% TFA as the mobile phase, adopt AB phase gradient mixing, and use a SHIMADZUInertsil ODS-SP chrom...

Embodiment 2

[0049] Embodiment 2 Antimicrobial Peptide In Vitro Bacteriostatic Activity Hemolytic Activity Determination

[0050] Bacteria were cultured overnight at 37°C in a 96-well plate with Mueller Hinton medium, and the final concentration of bacteria in each well was 5×10 5 CFU / ml. After the polypeptide was added, it was cultured at 37°C for 24 hours, and then the minimum inhibitory concentration (MIC) was determined by the microdilution method. MIC was defined as the lowest concentration of the polypeptide that killed all bacteria. The results showed that the antimicrobial peptides are effective against Gram-negative bacteria such as Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Gram-positive bacteria such as Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecalis, vine Both Micrococcus flavum and Bacillus subtilis had good antibacterial activity. The specific results are shown in Table 2 and Table 3 below.

[0051] The hemolytic activity of t...

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Abstract

The invention provides a group of antibacterial peptides and application thereof. The antibacterial peptide consists of 29 amino acids, and the N terminal and C terminal of the antibacterial peptide are respectively acetylized and amidated. The antibacterial peptide has the advantages that the antibacterial activity is higher, and the hemolytic activity is lower; the pathogenic bacteria in the organism can be more specifically killed.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to an antibacterial peptide and its application. Background technique [0002] The discovery and large-scale clinical application of antibiotics have enabled people to effectively deal with the invasion of pathogenic microorganisms into the human body, improving people's quality of life and prolonging their average life expectancy. However, with the abuse of antibiotics, clinical tests have found that the types of bacterial drug-resistant strains are increasing, and the clinical detection rate is also increasing. In order to suppress or kill drug-resistant bacteria, people are forced to develop new antibiotics to deal with. However, it is becoming more and more difficult to discover new antibiotics. Currently, only three antibiotics with new structures have been approved for clinical application. In addition, with the large-scale application of veterinary antibiotics in liv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00A61K8/64A61Q19/10A61Q5/02A61Q5/12A61Q11/00A61K38/16A61P31/04A61L15/46A01N47/44A01P1/00
CPCA01N47/44A61K8/64A61K38/00A61L15/46A61L2300/252A61L2300/404A61P31/04A61Q5/02A61Q5/12A61Q11/00A61Q19/10C07K14/00
Inventor 衣同辉李淑艳郭红艳
Owner QIQIHAR MEDICAL UNIVERSITY
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