Check patentability & draft patents in minutes with Patsnap Eureka AI!

The snp site in the promoter region of hoxa13 gene and its application in the detection of pig intramuscular fat content

A technology of intramuscular fat and content, applied in the field of molecular biology, can solve the problems of affecting muscle light reflection value, cooking loss, muscle flavor effect, etc.

Active Publication Date: 2022-06-21
NANJING AGRICULTURAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the intramuscular fat content will also affect the light reflection value and cooking loss of the muscle, which has a greater impact on the flavor of the muscle

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • The snp site in the promoter region of hoxa13 gene and its application in the detection of pig intramuscular fat content
  • The snp site in the promoter region of hoxa13 gene and its application in the detection of pig intramuscular fat content
  • The snp site in the promoter region of hoxa13 gene and its application in the detection of pig intramuscular fat content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Determination of intramuscular fat content of samples

[0034] 230 scalpel Tran × Duroc × Yorkshire × Dabai quaternary hybrid commercial pigs were used, and the experimental animals were provided by Wen's Group and collected from the large-scale slaughterhouse of Su Shi Group. All experimental animals were raised under the same conditions, and the longissimus dorsi muscle was quickly collected after slaughter, and the following steps were carried out respectively:

[0035] (1) Disperse 9cm medium-speed quantitative filter paper in a clean enamel tray and bake at 105°C for 2h. Weigh with an electronic analytical balance, and the weight of the filter paper is recorded as W1;

[0036] (2) Take about 2-3 g of the longissimus dorsi muscle sample, chop it into pieces, wrap it with dried filter paper and weigh it, and record the wet weight of the paper wrap as W2;

[0037] (3) Spread out the paper package in the enamel plate, beware of contact, bake in the oven at 65°C fo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a SNP site for detecting fat content in pork muscle, the SNP site is located at 1311 bp upstream of the transcription initiation site of pig HOXA13 gene, and the genotype of the SNP site is GG, GT or TT . Therefore, this SNP was named g.‑1311G>T. The invention also provides a method for detecting the intramuscular fat content of pork, which extracts the DNA of the pork sample to be detected, then types the amplified fragment, and judges the intramuscular fat content of the pork sample according to the typing result. The present invention obtains a method for detecting the intramuscular fat content of pigs by studying the correlation between the key gene for muscle development HOXA13 and the intramuscular fat content. , conveniently and quickly determine the fat content of pork muscle and lay the foundation, which is of great significance to the cultivation of pigs with good meat quality.

Description

technical field [0001] The invention belongs to the field of molecular biology, and particularly relates to a SNP site in the promoter region of a pig HOXA13 gene and its application. Background technique [0002] With the improvement of people's living standards, the quality of meat has gradually become the focus of people's attention. Intramuscular fat is white fat distributed in muscle tissue, and its content can change the quality of muscle in many aspects such as muscle tenderness, marbling, and muscle flavor. With the increase of intramuscular fat content, the thinner the muscle fibers, the less connective tissue between the muscle fibers, the lower the shear force of the muscle, and the better the tenderness of the meat. The higher the intramuscular fat content, the higher the muscle marbling score. The higher the intramuscular fat content, the richer the lipids on the muscle surface. The film formed by the fat on the muscle surface can alleviate the degree of calpa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 韦伟陈杰张立凡赵月雷林祥盛宋逸凡
Owner NANJING AGRICULTURAL UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com