Carbon quantum dot-molecular imprinting fluorescence sensor material for detecting chloramphenicol and preparation method and application thereof

A fluorescent sensor, carbon quantum dot technology, applied in the fields of analyzing materials, chemical instruments and methods, and material analysis by optical means, can solve the problems of CdTe quantum dot toxicity, heavy metal ion leakage, slow response speed, etc., and achieve excellent water quality. - Dispersibility and stability, sensitive detection, low cost effect

Inactive Publication Date: 2019-08-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, common metal quantum dots such as CdSe, CdTe, etc. contain highly toxic heavy metals, and may cause environmental pollution due to the leakage of heavy metal ions during use. Therefore, although they have excellent optical properties, they cannot Environmentally unfriendly and biologically unfriendly m

Method used

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  • Carbon quantum dot-molecular imprinting fluorescence sensor material for detecting chloramphenicol and preparation method and application thereof
  • Carbon quantum dot-molecular imprinting fluorescence sensor material for detecting chloramphenicol and preparation method and application thereof
  • Carbon quantum dot-molecular imprinting fluorescence sensor material for detecting chloramphenicol and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of carbon quantum dot-molecularly imprinted fluorescent sensor

[0032] 1. Preparation of nitrogen-doped carbon quantum dots

[0033] Add 12mmol urea and 4mmol citric acid into 20mL of water, ultrasonically dissolve it, seal it into a 50mL reaction kettle, and heat at 160°C for 4h. After cooling, add an appropriate amount of acetone to break the emulsification, centrifuge at 5000r / min for 5min, and vacuum dry the precipitate to obtain solid carbon quantum dots.

[0034] 2. Synthesis of Molecularly Imprinted Fluorescent Sensor Based on Carbon Quantum Dots

[0035] Add 7.50 mL of cyclohexane, 1.80 mL of n-hexanol and 1.77 mL of Triton X-100 into a 100 mL Erlenmeyer flask with a stopper, and stir magnetically for 20 min at a speed of 800 r / min. Add 1 mL of 1 mg / mL carbon quantum dot solution and stir for 20 min. Add 0.80 mmol tetraethoxysilane and 60 μL ammonia water and stir for 2 h. A prepolymerization solution consisting of 0.20 mmol of 3-amino...

Embodiment 2

[0038] Example 2 Characterization and Performance Evaluation of Carbon Quantum Dot-Molecularly Imprinted Fluorescent Sensor

[0039] Such as figure 1 As shown, A and B are transmission electron micrographs of carbon quantum dots, and C and D are transmission electron micrographs of carbon quantum dots-molecularly imprinted fluorescent sensors. From figure 1 In A, it can be seen that the size of carbon quantum dots is about 2-5nm and has a lattice structure. sensor material ( figure 1 C) in C) has a larger size of about 10nm, which is because the molecular imprinted layer is wrapped on the surface of the carbon quantum dots. and in figure 1 In D, it can be seen that there is a group of darker color in the sensor microsphere, which is the wrapped carbon quantum dot. exist figure 1 B in can see the aggregation state of carbon quantum dots, and figure 1 The sensor material of C in C shows better dispersion. This is because the strong van der Waals force between the surface...

Embodiment 3

[0042] Example 3 Application of carbon quantum dot-molecularly imprinted fluorescent sensor in the detection of chloramphenicol

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Abstract

The invention discloses a carbon quantum dot-molecular imprinting fluorescence sensor material for detecting chloramphenicol and a preparation method and application thereof. Carbon quantum dots are taken as a fluorescent carrier, a molecularly imprinted membrane is taken as an enrichment container, and the fluorescent molecularly imprinted material with a core-shell structure is synthesized, so that the specific detection of chloramphenicol can be realized. An inverse microemulsion method is adopted, firstly, an inverse microemulsion system is constructed, chloramphenicol is used as a template molecule in a water phase, and carbon quantum dots are used as a carrier to synthesize a molecularly imprinted membrane on the surface of the water phase. The prepared material is nano-scale microspheres and has excellent water-dispersion and stability. The material has good sensitivity to a target substance chloramphenicol, is high in detection speed, high in specific selectivity, high in chemical stability and low in cost, and has a good application prospect in chloramphenicol detection.

Description

technical field [0001] The invention relates to a carbon quantum dot-molecularly imprinted fluorescent sensor material for detecting chloramphenicol, a preparation method and application thereof, and belongs to the technical field of fluorescent sensor material preparation and food safety detection methods. Background technique [0002] As a broad-spectrum antibiotic with strong antibacterial ability, chloramphenicol has been widely used, but due to its serious side effects, such as bone marrow hematopoietic inhibition toxicity, genotoxicity, gray baby syndrome, etc., it has become the first An antibiotic banned for use in edible animals. The difficulty in determining chloramphenicol in food lies in the extremely low concentration of 1-10 μg / kg in various samples with complex matrices. Therefore, analyzes involving chloramphenicol monitoring require novel, fast and accurate cleanup and enrichment methods. [0003] The traditional detection methods of chloramphenicol includ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432B01J20/268B01J20/3085G01N21/6489G01N33/02G01N2021/7786B01L3/52B01L2200/12B01L2200/18B01L2300/123
Inventor 于航陈诗诗谢云飞郭亚辉成玉梁姚卫蓉
Owner JIANGNAN UNIV
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