Method and apparatus for measuring tissue topography and optical parameters based on spatial frequency domain imaging

A technology of optical parameters and organization, applied in the field of optics and measurement, can solve problems such as data distortion, restriction of real-time measurement, interference, etc.

Active Publication Date: 2019-08-06
HUAZHONG AGRI UNIV
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Problems solved by technology

However, the traditional spatial frequency domain imaging uses the three-phase shift method for demodulation, that is, it is necessary to collect diffuse reflection images of three different phases at the same modulation frequency to analyze the modulation depth at the modulation frequency; and to obtain the absorption coefficient and the reduced Scattering coefficient, it is necessary to measure the modulation depth at two or more modulation frequencies, and multiple imaging of the sample to be tested is required, which restricts the real-time performance of the measurement
In addition, when the surface height of the tissue to be measured is inconsistent, the structured light projected onto the surface of the tissue does not satisfy the sinu

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  • Method and apparatus for measuring tissue topography and optical parameters based on spatial frequency domain imaging
  • Method and apparatus for measuring tissue topography and optical parameters based on spatial frequency domain imaging
  • Method and apparatus for measuring tissue topography and optical parameters based on spatial frequency domain imaging

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Embodiment 1

[0049] Refer to attached figure 1 , figure 1 The straight fringe pattern on the left side of the middle is a schematic diagram of the incident sinusoidal modulated light, and the curved fringe pattern on the host computer 3 is a schematic diagram of the image captured by the CCD; the diffuse reflectance of the standard diffuse reflection plate 6 is uniform and known, and the tissue to be tested Body samples 7 are animal skin, liver, agricultural products, fruits and vegetables, and the like.

[0050] A method for measuring tissue morphology and optical parameters based on spatial frequency domain imaging technology, the steps of which are:

[0051] K1. The projector 1 and the CCD camera 2 are located at the same height above the reference plane 8, the optical axis of the projector 1 is perpendicular to the reference plane 8, the optical axis of the CCD camera 2 intersects with the normal direction of the reference plane 8 to form an angle A, Angle A=15°, adjust the height an...

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Abstract

The invention provides a method for measuring tissue topography and optical parameters based on spatial frequency domain imaging. The method comprises the following steps: 1, generating modulated light having a certain spatial frequency by a light source; 2, carrying out system calibration by using a reference plane; 3, projecting the modulated light on a to-be-measured tissue and collecting a reflected light image scattered by a sample by a camera; 4, acquiring a three-dimensional surface shape by using the Fourier contouring way; 5, correcting image illumination based on a height and an angle; 6, carrying out Fourier transform row by row, decomposing DC and AC spectral components, and then carrying out inverse Fourier transform to obtain DC and AC component images; and 7, carrying out matching fitting to obtain optical parameters. According to the provided method, surface shape and optical parameter information can be obtained only by one imaging; and since the illuminance error caused by the complex surface shape is corrected, the measurement speed is increased and the precision is improved.

Description

technical field [0001] The invention belongs to the field of optics and measurement technology, and more specifically relates to a method for measuring tissue morphology and optical parameters based on spatial frequency domain imaging, and also relates to a measuring device for measuring tissue morphology and optical parameters based on spatial frequency domain imaging , suitable for measuring the absorption coefficient and reduced scattering coefficient of biological tissue. Background technique [0002] The Chinese patent with the publication number CN105510253A "Apparatus and method for detecting optical characteristics of agricultural product tissue by spatial frequency domain imaging" discloses the spatial frequency domain imaging technology, by projecting sinusoidal modulated light with a certain spatial frequency onto the tissue sample to be tested , the camera collects diffuse light illumination images scattered by tissue, using a specific light transmission model, s...

Claims

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Application Information

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IPC IPC(8): G01B11/24
CPCG01B11/24
Inventor 丁驰竹谭佐军陈建军程其娈
Owner HUAZHONG AGRI UNIV
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