A kind of hydroponic multiplication method of tissue culture seedlings

Abstract

The invention relates to a method for hydroponic proliferation of tissue culture seedlings of thesium chinense Turcz.. The method comprises the steps of firstly, taking strong terminal buds of thesiumchinense Turcz., cleaning and disinfecting the terminal buds, and then inoculating the terminal buds into an MS culture medium for culture; secondly, transferring the terminal buds into a cluster budinduction culture medium for culture, inoculating generated adventitious buds into a proliferation culture medium for seedling strengthening proliferation culture, and obtaining the tissue culture seedlings of thesium chinense Turcz.; selecting the strong tissue culture seedlings dark green in color, transferring the tissue culture seedlings into an artificial climate chamber, opening a cover forhardening of the seedlings to obtain hydroponic mother seedlings of thesium chinense Turcz., planting the hydroponic mother seedlings into a hydroponic tank filled with a proliferation nutrient solution, and performing hydroponic proliferation culture to obtain the proliferated tissue culture rootless seedlings of thesium chinense Turcz.. The hydroponic seedlings obtained by means of the method have good quality and can be spread flat for air-drying for 2 days to serve as a raw material for extraction of kaempferol 3-glucorhamnoside, extraction of kaempferol 3-glucorhamnoside on the basis oftissue culture seedlings and wild herba thesium chinense Turcz. can be completely replaced, and the problem of resource shortage of thesium chinense Turcz. is solved. Compared with existing rootless seedling proliferation technologies of thesium chinense Turcz., the method has the advantages of large proliferation coefficient and low production cost, and large-scale production of the tissue culture rootless seedlings of thesium chinense Turcz. is facilitated.

Description

technical field [0001] The invention relates to a method for hydroponic multiplication of Thyme chinensis tissue culture seedlings, belonging to the technical field of plant cultivation. Background technique [0002] Thyme (Thesium chinense Turcz.) is a perennial semi-parasitic herb of Sandalaceae. Its dry whole herb is a common Chinese herbal medicine in my country. It has significant anti-inflammatory, analgesic, heat-relieving and broad-spectrum antibacterial effects. Spectrum antibacterial herbal medicine. The content of thymelin in thyme grass is relatively high, and it is easy to separate and extract, so it is a good raw material for the production of thymelin. Thyme officinalis is a semi-parasitic plant, and its cultivation is relatively difficult. At present, most of the raw materials of thyme officinalis depend on wild resources, but with the deterioration of the ecological environment and excessive harvesting, the wild resources of thyme officinalis are almost exha...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the shortage of wild thyme grass resources in the prior art and the problems of frequent transfer of thyme grass tissue culture seedlings, easy pollution and high growth cost in the process of producing thyme grass by tissue culture, and provide a The hydroponic multiplication method of Thyme chinensis tissue culture seedlings, the method multiplication speed is fast, the production cycle is short, not subject to seasonal restrictions, low cost, can realize the large-scale production of Thyme grass tissue culture seedlings, the obtained Thyme grass multiplication seedlings Stable quality, good quality

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