Three-dimensional simulation culture system for stem cells

A technology of three-dimensional simulation and culture system, which is applied in the field of three-dimensional simulation culture system of stem cells, can solve the problems of complex operation, poor quality, low efficiency, etc., and achieve the effect of high culture efficiency, reduced complexity and high quality

Inactive Publication Date: 2019-08-16
哈尔滨工大国际干细胞工程研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a three-dimensional simulation culture system for stem cells in order to solve the problems of complex operation, low efficiency, low yield and poor quality in the existing in vitro culture of stem cells

Method used

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  • Three-dimensional simulation culture system for stem cells

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specific Embodiment approach 1

[0021] Specific implementation mode one: as figure 1 As shown, a three-dimensional simulation culture system for stem cells in this embodiment includes: a stem cell nest 1, a first peristaltic pump 2 in the heart area, a lung area 3, a kidney area 4, a spleen area 5, a stomach area 6, a liver area 7, First fluid pressure gauge 8, first pressure regulating valve 9, liquid level gauge 10, three-way valve 11, first control valve 12, thermostat 13, temperature measuring instrument 14, oxygen / carbon dioxide measuring instrument 15, PH measuring instrument 16. Nutrient component measuring instrument 17, second control valve 18, second peristaltic pump 19, third peristaltic pump 20, urea / uric acid / inorganic salt measuring instrument 21, fourth peristaltic pump 22, third control valve 23, second Fluid pressure gauge 24, second pressure regulating valve 25, fourth control valve 26, fifth control valve 27, fifth peristaltic pump 28, sixth control valve 29, gas permeable membrane 30, dia...

specific Embodiment approach 2

[0049] Specific Embodiment 2: This embodiment differs from Specific Embodiment 1 in that the transportation involving liquid or gas is connected through a sterile silicone tube. Others are the same as in the first embodiment.

[0050] In this embodiment, the inner diameter of the sterile silicone tube is 4 mm, and the outer diameter is 6 mm.

specific Embodiment approach 3

[0051] Embodiment 3: The difference between this embodiment and Embodiment 1 is that the stem cell nest 1 is filled with a gel, and the stem cells are placed in the gel. Others are the same as in the first embodiment.

[0052] The initial concentration of stem cells in the stem cell nest 1 in this embodiment is 5×10 5 cells / mL.

[0053] The gel in this embodiment is based on the composition characteristics of the extracellular matrix of the stem cells to be cultured, selects a gel with high biocompatibility with the living environment of the stem cells, fully mixes and wraps around the stem cells, and provides a good supply for the subsequent stem cell culture. Protect and provide nutrients for stem cell growth and excrete metabolic waste.

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Abstract

The invention discloses a three-dimensional simulation culture system for stem cells, and belongs to the technical field of biology. The invention solves the problems of complex operation, low efficiency, small yield and poor quality in an existing in-vitro culture of stem cells. The three-dimensional simulation culture system for the stem cells comprises a stem cell nest, a heart region first peristaltic pump, a lung region, a kidney region, a spleen region, a stomach region, a liver region, a first fluid pressure gauge, a first pressure regulating valve, a liquid level meter, a three-way valve, a first control valve, a temperature regulator, a thermometer, an oxygen/carbon dioxide measuring instrument, a PH measuring instrument, a nutritional ingredient measuring instrument, a second control valve, a second peristaltic pump, a third peristaltic pump, a urea/uric acid/inorganic salt measuring instrument, a fourth peristaltic pump, a third control valve, a second fluid pressure gauge,a second pressure regulating valve, a fourth control valve, a fifth control valve, a fifth peristaltic pump, a sixth control valve, a gas permeable membrane, a dialysis membrane, a central control platform, a cloud data center and an electromagnetic field. According to the three-dimensional simulation culture system for the stem cells, the stem cells with higher quality and quantity can be cultured, the stability is good, and the treatment effect of the stem cells is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a three-dimensional simulation culture system of stem cells. Background technique [0002] Stem cells are a kind of primitive and underdifferentiated cells, which have the potential function of regenerating various tissues and organs and the ability of self-replication and renewal. Stem cell culture research is of great significance to the development of organ repair and renewal, disease treatment, bioengineering and other fields. [0003] The in vitro culture of stem cells has strict requirements on environmental conditions, equipment structure, and medium components. The existing stem cell culture method is mainly carried out manually, the operation is complicated, the efficiency is low, and the expansion degree of the cells is limited. The cells often lose the ability to proliferate and differentiate after long-term division, and the quantity and quality of stem cells...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/34C12M1/36C12M1/38
CPCC12M21/08C12M41/26C12M41/12C12M41/34C12M41/32C12M41/00C12M41/48C12N2513/00
Inventor 高会军潘惠惠杨宪强于兴虎金飞虎华子昂
Owner 哈尔滨工大国际干细胞工程研究院有限公司
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