Three-dimensional simulation culture system for stem cells
A technology of three-dimensional simulation and culture system, which is applied in the field of three-dimensional simulation culture system of stem cells, can solve the problems of complex operation, poor quality, low efficiency, etc., and achieve the effect of high culture efficiency, reduced complexity and high quality
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specific Embodiment approach 1
[0021] Specific implementation mode one: as figure 1 As shown, a three-dimensional simulation culture system for stem cells in this embodiment includes: a stem cell nest 1, a first peristaltic pump 2 in the heart area, a lung area 3, a kidney area 4, a spleen area 5, a stomach area 6, a liver area 7, First fluid pressure gauge 8, first pressure regulating valve 9, liquid level gauge 10, three-way valve 11, first control valve 12, thermostat 13, temperature measuring instrument 14, oxygen / carbon dioxide measuring instrument 15, PH measuring instrument 16. Nutrient component measuring instrument 17, second control valve 18, second peristaltic pump 19, third peristaltic pump 20, urea / uric acid / inorganic salt measuring instrument 21, fourth peristaltic pump 22, third control valve 23, second Fluid pressure gauge 24, second pressure regulating valve 25, fourth control valve 26, fifth control valve 27, fifth peristaltic pump 28, sixth control valve 29, gas permeable membrane 30, dia...
specific Embodiment approach 2
[0049] Specific Embodiment 2: This embodiment differs from Specific Embodiment 1 in that the transportation involving liquid or gas is connected through a sterile silicone tube. Others are the same as in the first embodiment.
[0050] In this embodiment, the inner diameter of the sterile silicone tube is 4 mm, and the outer diameter is 6 mm.
specific Embodiment approach 3
[0051] Embodiment 3: The difference between this embodiment and Embodiment 1 is that the stem cell nest 1 is filled with a gel, and the stem cells are placed in the gel. Others are the same as in the first embodiment.
[0052] The initial concentration of stem cells in the stem cell nest 1 in this embodiment is 5×10 5 cells / mL.
[0053] The gel in this embodiment is based on the composition characteristics of the extracellular matrix of the stem cells to be cultured, selects a gel with high biocompatibility with the living environment of the stem cells, fully mixes and wraps around the stem cells, and provides a good supply for the subsequent stem cell culture. Protect and provide nutrients for stem cell growth and excrete metabolic waste.
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