The invention discloses a three-
dimensional simulation culture 
system for stem cells, and belongs to the technical field of 
biology. The invention solves the problems of complex operation, low efficiency, small yield and 
poor quality in an existing in-vitro culture of stem cells. The three-
dimensional simulation culture 
system for the stem cells comprises a 
stem cell nest, a heart region first 
peristaltic pump, a 
lung region, a 
kidney region, a 
spleen region, a 
stomach region, a liver region, a first 
fluid pressure gauge, a first pressure regulating valve, a liquid level meter, a three-way valve, a first control valve, a temperature 
regulator, a 
thermometer, an 
oxygen / 
carbon dioxide measuring instrument, a PH 
measuring instrument, a nutritional ingredient 
measuring instrument, a second control valve, a second 
peristaltic pump, a third 
peristaltic pump, a 
urea / 
uric acid / inorganic salt measuring instrument, a fourth peristaltic pump, a third control valve, a second 
fluid pressure gauge,a second pressure regulating valve, a fourth control valve, a fifth control valve, a fifth peristaltic pump, a sixth control valve, a gas permeable membrane, a 
dialysis membrane, a central control platform, a 
cloud data center and an 
electromagnetic field. According to the three-
dimensional simulation culture 
system for the stem cells, the stem cells with higher quality and quantity can be cultured, the stability is good, and the 
treatment effect of the stem cells is improved.