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Epigenetic modification method of Enteromorpha-derived fungi and its secondary metabolite

A modification method, fungal technology, applied in the direction of microorganism-based methods, fungi, metabolic diseases, etc., can solve the problems of poor understanding, and achieve the effects of preventing hyperglycemia, easy absorption, and enhancing the degree of acetylation

Inactive Publication Date: 2019-08-16
嘉兴市爵拓科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In contrast, little is known about the mechanisms regulating the biosynthesis of secondary metabolites at the molecular level

Method used

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  • Epigenetic modification method of Enteromorpha-derived fungi and its secondary metabolite
  • Epigenetic modification method of Enteromorpha-derived fungi and its secondary metabolite
  • Epigenetic modification method of Enteromorpha-derived fungi and its secondary metabolite

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Experimental program
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Effect test

preparation example Construction

[0030] The preparation method of above-mentioned secondary metabolite is:

[0031] 1) the fermentation broth is filtered through cheesecloth to separate the filtrate and mycelia, the filtrate is extracted three times with an equivalent volume of ethyl acetate to obtain an ethyl acetate solution, the mycelium is extracted three times with 80% acetone, the acetone solution is concentrated under reduced pressure, and then Extract with ethyl acetate three times to obtain an ethyl acetate solution, combine the above two ethyl acetate solutions together, and concentrate under reduced pressure to obtain an ethyl acetate solution extract;

[0032] 2) The ethyl acetate extract was eluted step by step by vacuum silica gel column chromatography, and the eluent was petroleum ether-CH 2 Cl 2 (1:1 and 0:1) and CH 2 Cl 2 - Yield gradient of MeOH (100% ~ 0%) 6 main fractions (fraction 1 ~ 6), fraction 4 was further purified by Sephadex LH-20 (MeOH) to obtain fractions 4.2, 4.3, 4.4, fracti...

Embodiment 1

[0035] A preparation method for the appearance modification method of enteromorpha-derived fungi, comprising:

[0036] 1) Fungal material: The fungal strain Aspergillus terreus OUCMDZ-2739 was isolated from Enteromorpha samples from Shilaoren Beach in Qingdao, and the Enteromorpha samples were washed with sterile seawater, 75% ethanol and sterile water in sequence. Then, the samples were crushed with a mortar and pestle, and then stored in PDA medium (containing 200 g of potato extract, 20 g of glucose, 15 g of agar, and 1 L of seawater per liter) containing 100 mg / mL chloramphenicol at 28 ° C. After cultured until a single strain appeared, the strain was transferred to another PDA medium and stored at 4°C. Through multiphase taxonomic studies, colony morphology and 18S rRNA sequence analysis, and establishment of strain development trees, it was identified as soil Aspergillus OUCMDZ-2739;

[0037] 2) Expansion cultivation: inoculate the Enteromorpha-derived fungus Aspergillu...

Embodiment 2

[0046] A preparation method for the appearance modification method of Enteromorpha-derived fungi, comprising:

[0047] 1) Fungal material: The fungal strain Aspergillus terreus OUCMDZ-2739 was isolated from Enteromorpha samples from Shilaoren Beach in Qingdao, and the Enteromorpha samples were washed with sterile seawater, 75% ethanol and sterile water in sequence. Then, the samples were crushed with a mortar and pestle, and then stored in PDA medium (containing 200 g of potato extract, 20 g of glucose, 15 g of agar, and 1 L of seawater per liter) containing 100 mg / mL chloramphenicol at 28 ° C. After cultured until a single strain appeared, the strain was transferred to another PDA medium and stored at 4°C. Through multiphase taxonomic studies, colony morphology and 18S rRNA sequence analysis, and establishment of strain development trees, it was identified as soil Aspergillus OUCMDZ-2739;

[0048] 2) Expansion cultivation: inoculate the Enteromorpha-derived fungus Aspergillu...

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Abstract

The invention provides an epigenetic modification method of Enteromorpha-derived fungi and its secondary metabolite, which belong to the technical field of a microbe medicine. The epigenetic modification method comprises the following steps: performing enlargement culture: inoculating the Enteromorpha-derived fungi in a culture medium for enlargement culture to obtain a fungal seed liquid; and epigenetic modification regulation: inoculating the fungal seed liquid to a fermentation liquid sterilized by a pulsed electric field and ultraviolet rays to obtain an marine fungus with epigenetic modification. the secondary metabolite of the Enteromorpha-derived fungi obtained by the epigenetic modification method mainly include a compound I, a compound II, a compound III, and a compound IV. The epigenetic modification method of the invention can promote the expression of pluripotency-related genes of the Enteromorpha-derived fungi, increases the diversity of the secondary metabolite, promotesthe proliferative activity of the Enteromorpha-derived fungi, increases the growth rate of the Enteromorpha-derived fungi, and prolong the log phase, and increases the yield of secondary metabolite. The compound III can inhibit the alpha-glucosidase activity of a body.

Description

technical field [0001] The invention belongs to the technical field of microbial medicines, and in particular relates to a method for modifying the appearance of enteromorpha-derived fungi and secondary metabolites thereof. Background technique [0002] In the nearly one hundred years of research on terrestrial microorganisms, a large number of natural products with diverse chemical structures and significant biological activities have been discovered, which has greatly promoted the development of biotin drugs, such as: penicillin, vancomycin, streptomycin, etc. More and more attention to microorganisms naturally leads to repeated research on microorganisms, repeated development of known compounds, and a lower chance of discovering new compounds. Therefore, people began to pay attention to marine-derived microorganisms with larger microbial reserves and more diverse species. Due to their unique living environment (high pressure, high salt, low temperature, oligotrophic), ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C07D407/06C07D407/14C07D311/58C07D307/80A61P3/10C12R1/66
CPCA61P3/10C07D307/80C07D311/58C07D407/06C07D407/14C12N1/14
Inventor 孙坤来张译文宫凯凯赵成英
Owner 嘉兴市爵拓科技有限公司
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