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Hemolytic agent used for leukocyte classification

A technology for leukocyte classification and hemolytic agent, applied in the field of blood cell analysis and detection, can solve the problems of complicated operation of leukocyte classification technology, and achieve the effect of reducing the cost and complexity of research and development, and reducing the harm to the environment.

Pending Publication Date: 2019-08-16
SHENZHEN DYMIND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The object of the present invention is to provide a new hemolytic agent for leukocyte classification, which can differentiate leukocytes into lymphocytes (Lym), monocytes (Mon), neutrophils (Neu) and eosinophils (Eos). ) four subpopulations, no need to add any dyes, reduce the complexity of reagent and instrument development, and aim to solve the problem of complex operation in the existing technology of white blood cell classification

Method used

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  • Hemolytic agent used for leukocyte classification
  • Hemolytic agent used for leukocyte classification
  • Hemolytic agent used for leukocyte classification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070]

[0071] Take 25 clinical venous whole blood samples, and use the hemolytic agent configured in Example 1 on the Dimai DF50 hematology analyzer for experiments, and use the original Mindray hemolytic agent for experiments on the Mindray BC-5180 hematology analyzer. The two results are processed in Excel. The test result on Mindray BC-5180 is used as the ordinate, and the test result of Example 1 is used as the abscissa, as Figure 1a-Figure 1d As shown, it can be seen from the figure that the result has a good linear relationship with the test result on Mindray BC-5180.

Embodiment 2

[0073]

[0074]

[0075] Take 25 clinical venous whole blood samples, use the hemolytic agent configured in this example 2 on the Dimai DF50 hematology analyzer to perform the experiment, and at the same time perform the experiment on the Mindray BC-5180 hemolytic agent (using the original Mindray hemolytic agent) In the experiment, the two results were processed in Excel, and the test result on Mindray BC-5180 was used as the ordinate, and the test result of Example 2 was used as the abscissa. Such as Figure 2a-Figure 2d As shown, it can be seen from the figure that the result has a good linear relationship with the test result on Mindray BC-5180.

Embodiment 3

[0077]

[0078] Take 25 clinical venous whole blood samples, use the hemolytic agent configured in Example 3 on the Dimai DF50 hematology analyzer to conduct experiments, and at the same time perform the experiment on the Mindray BC-5180 hemolytic agent (using the original Mindray hemolytic agent) In the experiment, the two results were processed in Excel. The test result on Mindray BC-5180 was used as the ordinate, and the test result of Example 3 was used as the abscissa. Such as Figure 3a-Figure 3d As shown, it can be seen from the figure that the result has a good linear relationship with the test result on Mindray BC-5180.

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Abstract

The invention discloses a hemolytic agent used for leukocyte classification. The hemolytic agent used for the leukocyte classification comprises the following components: a cationic surface active agent, a non-ionic surface active agent, buffer solution with the pH value of 3-7, an osmotic pressure regulator, a dissolution promoting agent and an antibacterial agent. The hemolytic agent can enableleukocytes to be divided into four subgroups, namely lymphocytes, mononuclear leucocytes, neutrophile granulocytes and eosinophilic cells, on a DYMIND hematology cell analyzer. The hemolytic agent disclosed by the invention does not contain dyes, can classify the leukocytes by virtue of a laser scattering method without dyeing the leukocytes, reduces harm to the environment, reduces cost and complexity of research and development of reagents and instruments and is applicable to various medical institutions.

Description

Technical field [0001] The invention relates to the field of blood cell analysis and detection, in particular to a hemolytic agent for white blood cell classification. Background technique [0002] Normal peripheral blood leukocytes are generally divided into five categories, namely lymphocytes (Lym), monocytes (Mon), neutrophils (Neu), eosinophils (Eos), and basophils (Baso). In the field of clinical analysis, accurate detection of different types of white blood cells is very important for diagnosis and research. [0003] The current three-classification hematology analyzers on the market can only divide white blood cells into three subgroups of small cells, intermediate cells, and large cells by impedance method, and cannot accurately classify white blood cells, which has certain limitations in the field of clinical analysis. With the continuous improvement of science and technology in today's society, five-class blood cell analyzers generally use laser light scattering or a com...

Claims

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Application Information

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IPC IPC(8): G01N21/47
CPCG01N21/47
Inventor 舒家发崔晓磊余晓尘辛佩轩郑剑通其他发明人请求不公开姓名
Owner SHENZHEN DYMIND BIOTECH
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