Recurrent breeding method for widening genetic basis of two-line sterile line

Abstract

The invention relates to a recurrent breeding method for widening the genetic basis of a two-line sterile line. The recurrent breeding method comprises the following steps: i, taking dominant genic male sterility as a female parent, taking different germplasm as a male parent, collecting cross seeds and mixing the cross seeds at the same amount to form a dominant genic male sterility recurrent selection group C0 generation; ii, planting the CO generation, and carrying out genetic diversity detection; iii, circulating ii until the genetic diversity index is consistent with the average value; taking Shen 3S as a male parent to carry out hybridization and mixing to form a recurrent selection population on the background of Shen 3S, and selecting an excellent hybrid single plant; iv, carryingout background analysis through a chip, and keeping selfing to implement homozygosis; and v, after multiple generations, eliminating and selecting, and finally preferably selecting one strain to carryout breeding. The parent material disclosed by the invention is rich in genetic background and relatively wide in source, and is homozygous for a photo-thermo-sensitive sterile gene in a selfing mode, and an improved line with an enlarged genetic basis is finally obtained by using conventional breeding methods such as fertility screening and combination configuration.

Description

technical field [0001] The invention relates to the field of agricultural breeding, in particular to a recurrent breeding method for broadening the genetic basis of two-line sterile lines. Background technique [0002] Since Shi Mingsong reported the discovery of light-temperature-sensitive male sterile rice in 1981, Chinese scientists have conducted a lot of research on the use of heterosis by two-line method in rice, and have made great achievements that have attracted worldwide attention. Now two-line hybrid rice has occupied a very important position in the utilization of rice heterosis in my country. Statistics from the National Rice Data Center (http: / / www.ricedata.cn / variety / ) show that up to now, there are 354 rice photothermosensitive male sterile lines that have been bred in the country, of which two lines have passed the examination and approval above the provincial level. There are 130 male sterile lines. Because the pedigree method of cross breeding is too nar...

AI Technical Summary

Problems solved by technology

Because the pedigree method of cross breeding is too narrow in the selection range, many useful genes are often lost, resulting in the narrowing of the genetic basis of the original hybrid population, resulting in a relatively single genetic background of the two-line sterile lines in my country, mainly Nongken 58S and Annong S The derivative line of -1 restricts the utilization of heterosis and the improvement of stress resistance

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