Method for preparing high-purity chlorogenic acid from stevia rebaudiana

A technology of stevia rebaudiana and chlorogenic acid, which is applied to the preparation of carboxylic acid esters, sugar derivatives, chemical instruments and methods, etc., can solve the problems of complex preparation process, low yield, and single source of chlorogenic acid extraction, and achieve operational Simple, realize the effect of comprehensive utilization

Active Publication Date: 2019-08-23
DONGTAI HIRYE BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Solve the problems of single extraction source of chlorogenic acid, complex preparation process, use of a large amount of organic solvent, and low yield

Method used

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  • Method for preparing high-purity chlorogenic acid from stevia rebaudiana
  • Method for preparing high-purity chlorogenic acid from stevia rebaudiana
  • Method for preparing high-purity chlorogenic acid from stevia rebaudiana

Examples

Experimental program
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Effect test

Embodiment 1

[0039] 1kg of Stevia rebaudiana dry leaves were placed in a storage tank, extracted at 35°C with 3×4L of water, and the resulting Stevia rebaudiana extract was adjusted to pH 3.5 with 0.1mol / L hydrochloric acid. Add 120mL of 1wt% chitosan solution to the extract while stirring, and let stand for 50min to flocculate and remove impurities. The supernatant after flocculation and impurity removal is filtered with a plate frame, and the filtered supernatant is absorbed by PA6 resin, and the effluent is reserved for other uses; then the resin is eluted with 3 times the column volume of water, and the water eluate is collected for extraction Chlorogenic acid. Then, the PA6 resin was analyzed with a methanol solution with a volume concentration of 80%, and the alcohol analysis solution was collected. The alcohol analysis solution was further concentrated and dried for other use. Use PA6 resin to absorb and enrich the chlorogenic acid in the water eluate, then elute the resin with 3 t...

Embodiment 2

[0043] 1 kg of Stevia rebaudiana dry leaves were placed in a storage tank, extracted at 60°C with 3×2.5L of water, and the resulting Stevia rebaudiana extract was adjusted to pH 4.5 with 0.1mol / L acetic acid. Add 100 mL of 1% chitosan solution to the extract under stirring, and let stand for 20 minutes to flocculate and remove impurities. After deflocculation and impurity removal, the supernatant is filtered with a plate frame, and the filtered supernatant is absorbed by PA66 resin, and the effluent is reserved for other purposes; the resin is then eluted with 4 times the column volume of water, and the water eluent is used for extraction Chlorogenic acid. Then, analyze PA66 resin with 80% ethanol, and the alcohol analysis solution is further concentrated and dried and reserved for other use. Utilize PA66 resin to adsorb and enrich the chlorogenic acid in the water eluent, then, use 4 times of column volume water to elute the resin, and the chlorogenic acid content that colle...

Embodiment 3

[0045] 1kg of Stevia rebaudiana dry leaves were placed in a storage tank, extracted at 50°C with 3×3L of water, and the resulting Stevia rebaudiana extract was adjusted to pH 4.0 with 0.1mol / L hydrochloric acid. Add 120 mL of 1% chitosan solution to the extract under stirring, and let stand for 50 min to flocculate and remove impurities. The supernatant after deflocculation and impurity removal is filtered with a plate frame, and the filtered supernatant is adsorbed by LX-16 resin, and the effluent is reserved for other uses; then the resin is eluted with 5 times the column volume of water, and the eluent is used as Extract chlorogenic acid. Then use 85% methanol to analyze the LX-16 resin, and the alcohol analysis solution is further concentrated and dried for other use. Utilize LX-16 resin to absorb and enrich the chlorogenic acid in the water eluate, then, use 5 times column volume of water to elute the resin, collect and concentrate the water wash, and adjust the pH of th...

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Abstract

The invention discloses a method for preparing high-purity chlorogenic acid from stevia rebaudiana and belongs to the technical field of natural product chemicals. The method comprises the following steps: by taking stevia rebaudiana as a raw material, and water as an extraction medium, filtering and flocculating an extract liquid, purifying the extract liquid by using a polyamide resin or a polyamide-like resin, eluating with water as an eluent, and concentrating and crystallizing an eluation liquid, thereby obtaining a product that the mass content of chlorogenic acid is 98%. Meanwhile, in the process that the chlorogenic acid is purified is purified by using the resin, other collected components can be adopted to separate and extract other stevia rebaudiana polyphenol and stevioside. Tosum up, the method is capable of achieving preparation of high-purity chlorogenic acid from stevia rebaudiana as a raw material, is simple in process operation, free of organic solvent and environmental-friendly and safe, and the mass content of the chlorogenic acid in the product is up to 98% or above.

Description

technical field [0001] The invention relates to a method for preparing high-purity chlorogenic acid from stevia rebaudiana, in particular to a method for extracting high-purity chlorogenic acid from stevia rebaudiana by a water medium method, which belongs to the technical field of natural product chemistry. Background technique [0002] Chlorogenic acid, also known as coffee tannin, is a kind of chlorogenic acid. It is a depsipolic acid produced by caffeic acid and quinic acid (1-hydroxyhexahydrogallic acid). A phenylpropanoid compound produced by the shikimate pathway in the process. [0003] Chlorogenic acid is an important biologically active substance, which has great application value in the fields of medicine and health. Chlorogenic acid has certain pharmacological effects on digestion, blood and reproductive system, and has a wide range of antibacterial, anti-inflammatory, choleretic, and hemostatic effects; chlorogenic acid has strong antibacterial and antiviral ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C67/48C07C67/52C07C67/56C07C69/732C07H15/256C07H1/08A61K36/28
CPCC07C67/48C07C67/52C07C67/56C07H15/256C07H1/08A61K36/28C07C69/732
Inventor 朱理平何东生夏咏梅陈科材薛雪程双武樊晔
Owner DONGTAI HIRYE BIOTECH CO LTD
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