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Analysis method of lipid components in Fuzhuan tea

An analysis method and technology of Fu brick tea, applied in the direction of analysis material, material separation, measurement device, etc., can solve the problem of lack

Inactive Publication Date: 2019-08-27
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is a lack of systematic research on lipids in tea. Therefore, it is necessary to develop large-scale, highly sensitive, and highly specific methods for the analysis of lipids in tea.

Method used

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  • Analysis method of lipid components in Fuzhuan tea
  • Analysis method of lipid components in Fuzhuan tea
  • Analysis method of lipid components in Fuzhuan tea

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Embodiment Construction

[0029] The present invention will be described in further detail below in conjunction with specific examples, but the embodiments of the present invention include but are not limited to the scope indicated in the following examples.

[0030] The analysis method of lipid component in a kind of Fu brick tea provided by the invention, comprises the following steps successively:

[0031] Step 1. Sample preparation:

[0032] Take 50mg of tea powder of Heimao tea and Eucommia fermented Fu brick tea, add 300ul of methanol solution, 1ml of methyl tert-butyl ether solution, vortex for 1 hour, add 300ul of water, vortex for 30s, after high-speed centrifugation, take 500ul of the mixed liquid to freeze Dry; configure acetonitrile, isopropanol, water composite solution (acetonitrile: isopropanol: water = 65:30:5, volume ratio), add the above freeze-dried sample to 100ul solution, vortex, centrifuge, and take the supernatant 80ul to the injection vial as the analysis sample;

[0033] Ste...

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Abstract

The invention provides an analysis method of lipid components in Fuzhuan tea. The method is used for carrying out lipidomic analysis on a tea sample and carrying out structural identification on lipidsubstances. According to the invention, after tea powder of Fuzhuan tea is processed, a total ion flow diagram of lipid substance components in a Fuzhuan tea sample is obtained by adopting a tea lipidomic analysis method of ultra-high-phase liquid chromatography-quadrupole and orbital hydrazine high-resolution mass spectrometry combined UHPLC-Q Exactive MS, and original data of the total ion flowdiagram of the Fuzhuan tea sample is introduced into a lipid qualitative software to carry out structural identification on the lipid substances in the Fuzhuan tea, so that a theoretical basis is provided for regulation and control of the quality of the Fuzhuan tea.

Description

technical field [0001] The invention belongs to the field of detection and analysis of tea components, and in particular relates to an analysis method for lipid components in Fuzhuan tea. Background technique [0002] Fuzhuan tea belongs to post-fermentation tea, which is made from third- and fourth-grade tea as raw materials, and is processed through sieving, steaming, stacking, pressing, blooming and drying. Because of its unique aroma characteristics and irreplaceable health benefits, Fuzhuan tea has become a daily necessities for consumers in Northwest my country, and is known as "the mysterious tea on the ancient Silk Road in China". [0003] Aroma is an important factor in determining the quality of tea. The study found that lipid oxidative degradation is one of the four important ways to form aroma during tea processing. The "flowering" process creates the unique "fungus-flower fragrance" feature of Fuzhuan tea, and many aroma products related to lipid degradation a...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 赵燕妮席心茹赵瑞霞曾乔秦俊哲陈善纳林萍钟小荣张豪
Owner SHAANXI UNIV OF SCI & TECH
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