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Liquid chromatography-tandem mass spectrometry detection method for plurality of lipid-soluble vitamins in dried blood spots

A technology of fat-soluble vitamins and liquid chromatography, which is applied in the field of multivitamin detection, can solve the problems of no way to do multivitamin co-detection, high sampling requirements, low temperature conditions for sample transportation and storage, and achieve the detection method Simple, sensitive, repeatable, and highly specific effects

Inactive Publication Date: 2019-08-27
合肥谱佳医学检验实验室有限公司 +1
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Problems solved by technology

[0003] Most of the existing detection methods for fat-soluble vitamins are based on liquid blood samples, which have disadvantages such as high sampling requirements and low temperature conditions for sample transportation and storage.
Moreover, in the existing published literature, only one vitamin is detected, and there is no way to jointly detect multiple vitamins.

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  • Liquid chromatography-tandem mass spectrometry detection method for plurality of lipid-soluble vitamins in dried blood spots
  • Liquid chromatography-tandem mass spectrometry detection method for plurality of lipid-soluble vitamins in dried blood spots
  • Liquid chromatography-tandem mass spectrometry detection method for plurality of lipid-soluble vitamins in dried blood spots

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Embodiment Construction

[0034] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0035] The detection process of this embodiment is as follows:

[0036] 1. Preparation of dried blood film standard

[0037] 1. Preparation of standard product stock solution: Accurately weigh vitamin A and vitamin E standard products, dissolve them in methanol to make a stock solution with a concentration of 1 mg / mL, take 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 standard solutions, and use Dilute with methanol to make a stock solution with a concentration of 10 μg / mL.

[0038] 2. Preparation of mixed standard solution: Precisely pipette appropriate amount of vitamin A, 25-hydroxyvitamin D2, 25-hydroxyvitami...

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Abstract

The invention discloses a liquid chromatography-tandem mass spectrometry detection method for a plurality of lipid-soluble vitamins in dried blood spots. The method comprises the following steps: preparing 1 to 3 blood stain samples, placing the 1 to 3 blood stain samples in a centrifugal tube, adding first extraction liquid, and performing vortex treatment for 25 to 35 minutes; adding an internalstandard solution and second extraction liquid, and performing vortex treatment for 2 to 4 minutes; centrifuging the mixture, transferring a supernatant into another centrifugal tube, performing blowdrying with nitrogen gas, adding reconstitution fluid, performing vortex treatment for 2 to 3 minutes, and sampling the reconstitution fluid for analysis; injecting a dry blood spot sample solution prepared in the step (1) into a LC-MS / MS (Liquid Chromatograph-Tandem Mass Spectrometer), and recording a chromatogram; and acquiring the retention time and mass spectrum data of the plurality of lipid-soluble vitamins. The detection method has the advantages of easiness, sensitivity, rapidness, accuracy, high specificity and high repeatability, and can be used for detecting the plurality of lipid-soluble vitamins in the dried blood spots.

Description

technical field [0001] The invention relates to a detection method of multiple vitamins, in particular to a liquid chromatography tandem mass spectrometry detection method of multiple fat-soluble vitamins in dried blood tablets. Background technique [0002] Vitamins are divided into fat-soluble and water-soluble two categories. Fat-soluble vitamins (L-Vs) refer to vitamins that are insoluble in water but soluble in fat and organic solvents, including vitamins A, D, and E. [0003] Most of the existing detection methods for fat-soluble vitamins are based on liquid blood samples, which have disadvantages such as high sampling requirements and low temperature conditions for sample transportation and storage. Moreover, in the existing open literature, only one vitamin is detected, and there is no way to detect multiple vitamins together. [0004] Therefore, there is an urgent need for a method for the detection of fat-soluble vitamins that is simple in sampling, requires less...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/045
Inventor 江振作王旭徐元宏王兰芳朱俊王超华振祥
Owner 合肥谱佳医学检验实验室有限公司
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