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Screening detection method for pathogenic gene mutation of Parkinson's disease

A screening technology for Parkinson's disease, applied in biochemical equipment and methods, microbial measurement/inspection, biochemical instruments, etc., can solve the problems of reducing the accuracy and efficiency of screening and detection, hiding recessive genes, etc., and achieves convenience The effect of detection, ensuring accuracy and improving detection efficiency

Active Publication Date: 2019-09-03
THE SECOND AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, when the above-mentioned technical solutions are actually used, there are still many shortcomings. Once the temperature of the blood containing the mutation of the Parkinson's disease gene changes or the enzyme solution changes, it will lead to the hiding of the internal recessive gene, thereby reducing the screening rate. Detection accuracy and efficiency

Method used

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  • Screening detection method for pathogenic gene mutation of Parkinson's disease
  • Screening detection method for pathogenic gene mutation of Parkinson's disease
  • Screening detection method for pathogenic gene mutation of Parkinson's disease

Examples

Experimental program
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Effect test

Embodiment 1

[0037] according to Figure 1-7 A Parkinson's disease-causing gene mutation screening and detection device shown includes a detection box 1, a refrigerator room 2 is integrally formed above the detection box 1, and a PCR room 3 is provided on the side surface of the detection box 1, and the PCR chamber 3 is provided on the side surface of the detection box 1. A temperature adjustment knob 4 is provided on the front surface of the chamber 3, and a pumping assembly 5 is provided on one side of the PCR chamber 3, a stirring assembly 9 is provided on one side of the detection box 1, and multiple sets of baffles are installed inside the detection box 1 6. A storage tank 7 is arranged between multiple groups of the baffle plates 6, and a test tube 8 is installed inside the storage tank 7, and an enzyme injection component 10 is installed inside the refrigerator compartment 2;

[0038]The pumping assembly 5 includes a first fixed tube 51, a piston rod 52 is embedded under the first f...

Embodiment 2

[0053] according to Figure 1-7 A Parkinson's disease-causing gene mutation screening and detection device shown includes a detection box 1, a refrigerator room 2 is integrally formed above the detection box 1, and a PCR room 3 is provided on the side surface of the detection box 1, and the PCR chamber 3 is provided on the side surface of the detection box 1. A temperature adjustment knob 4 is provided on the front surface of the chamber 3, and a pumping assembly 5 is provided on one side of the PCR chamber 3, a stirring assembly 9 is provided on one side of the detection box 1, and multiple sets of baffles are installed inside the detection box 1 6. A storage tank 7 is arranged between multiple groups of the baffle plates 6, and a test tube 8 is installed inside the storage tank 7, and an enzyme injection component 10 is installed inside the refrigerator compartment 2;

[0054] The pumping assembly 5 includes a first fixed tube 51, a piston rod 52 is embedded under the first ...

Embodiment 3

[0069] according to Figure 1-7 A Parkinson's disease-causing gene mutation screening and detection device shown includes a detection box 1, a refrigerator room 2 is integrally formed above the detection box 1, and a PCR room 3 is provided on the side surface of the detection box 1, and the PCR chamber 3 is provided on the side surface of the detection box 1. A temperature adjustment knob 4 is provided on the front surface of the chamber 3, and a pumping assembly 5 is provided on one side of the PCR chamber 3, a stirring assembly 9 is provided on one side of the detection box 1, and multiple sets of baffles are installed inside the detection box 1 6. A storage tank 7 is arranged between multiple groups of the baffle plates 6, and a test tube 8 is installed inside the storage tank 7, and an enzyme injection component 10 is installed inside the refrigerator compartment 2;

[0070] The pumping assembly 5 includes a first fixed tube 51, a piston rod 52 is embedded under the first ...

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Abstract

The invention discloses a screening detection method for pathogenic gene mutation of Parkinson's disease, which relates to the technical field of disease gene mutation screening, the screening detection method comprises a detection box, a refrigerating chamber is integrally formed on the detection box, a PCR chamber is arranged on a side surface of the detection box, the front surface of the PCR chamber is provided with a temperature adjustment knob, a pumping assembly is disposed on one side of the PCR chamber, a stirring assembly is disposed on one side of the detection box, a plurality of sets of baffle plates are installed inside the detection box, the storage tanks are disposed between the plurality of sets of baffle plates, and the storage tanks are internally fitted with test tubes.The method mixes the enzyme liquid with the blood containing the pathogenic gene mutation of Parkinson's disease by different temperatures and different enzyme liquids, the pathogenic genes of Parkinson's disease in the blood is more active, which facilitates the detection of the workers and ensures the accuracy of screening tests, the detection efficiency is improved, and the method enables people to understand and effectively control the disease genes early.

Description

technical field [0001] The invention relates to the technical field of disease-causing gene mutation screening, and more specifically, the invention relates to a method for screening and detecting Parkinson's disease-causing gene mutation. Background technique [0002] Parkinson's disease, also known as "paralysis agitans", is a degenerative disease of the central nervous system, mainly due to pathological changes in the cells located in the "substantia nigra" of the midbrain, the synthesis of dopamine is reduced, and the function of inhibiting acetylcholine is reduced. , the excitatory effect of acetylcholine is relatively enhanced. The result of the imbalance between the two is "paralysis agitans". Parkinson's disease is the fourth most common neurodegenerative disease in the elderly. Among people ≥65 years old, 1% suffer from this disease; in people >40 years old, it is 0.4%. This disease can also occur in children onset during puberty or adolescence. [0003] Parki...

Claims

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Application Information

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IPC IPC(8): C12M1/40C12M1/38C12M1/02C12Q1/6883C12N15/11
CPCC12M21/18C12M41/12C12M27/02C12Q1/6883C12Q2600/156
Inventor 王运良花琳琳郑蔚
Owner THE SECOND AFFILIATED HOSPITAL OF ZHENGZHOU UNIV