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Double PCR primer set for simultaneous detection of MS-H vaccine strain and versatility and kit

A technology of MS-H and vaccine strains, applied in the direction of recombinant DNA technology, microorganisms, and methods based on microorganisms, etc., can solve the problems of decreased quality of broiler carcass, increased drug use for diseased groups, and increased waste rate, etc., achieving short detection cycles and strong Specificity and accuracy, practical effect

Active Publication Date: 2019-09-03
青岛英赛特生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After being infected with the disease, young chickens grow poorly, egg production of adult chickens decreases, air sac inflammation reduces the quality of broiler chicken carcasses, increases the abandonment rate, and increases the use of drugs for the diseased group, which can cause serious economic losses

Method used

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  • Double PCR primer set for simultaneous detection of MS-H vaccine strain and versatility and kit
  • Double PCR primer set for simultaneous detection of MS-H vaccine strain and versatility and kit
  • Double PCR primer set for simultaneous detection of MS-H vaccine strain and versatility and kit

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Experimental program
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Effect test

Embodiment 1

[0033] 1. Design specific primers

[0034] According to the sequence differences in GenBank, we used Primer 5 software to design a dual PCR primer set for simultaneous detection of Mycoplasma Synovium MS-H vaccine strain and universal type. The specific sequence is as follows:

[0035] Vaccine strain upstream primer (SEQ IDNO:1): TGTATAGTAACGCCCTTCGC

[0036] Downstream primer of vaccine strain (shown in SEQ ID NO: 2): CTTCTTGAAGGTGAAACCAA

[0037] Universal upstream primer (shown in SEQ ID NO: 3): CTATTAGCAGCTAGTGCAGTGG

[0038] Universal downstream primer (shown in SEQ ID NO: 4): ACCGATCCGCTTAATGCTCTTAC

[0039] 2. Nucleic acid extraction

[0040] Refer to the cotton swab DNA extraction kit instructions of Qingdao Insite Biotechnology Co., Ltd. to extract the DNA of various mycoplasma and store at -20°C.

[0041] 3. Specificity test of chicken Mycoplasma synovial sac MS-H vaccine strain and universal double PCR

[0042] This time, the nucleic acid of Mycoplasma gallisepticum and Mycoplasm...

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Abstract

The invention belongs to the technical field of biological detection, and relates to a double PCR primer set for simultaneous detection of a MS-H vaccine strain and versatility and a kit. The primer set comprises two pairs of primers, which are an upstream and downstream primer pair of a MS-H vaccine strain and a universal upstream and downstream primer pair; wherein, the upstream and downstream primer pair of the MS-H vaccine strain include an upstream primer of the vaccine strain shown as SEQ ID NO: 1, and a downstream primer of the vaccine strain shown as SEQ ID NO: 2, and the universal upstream and downstream primer pair include the universal upstream primer shown as SEQ ID NO: 3 and the universal downstream primer shown as SEQ ID NO: 4. The kit provided by the invention is used to detect the chicken mycoplasma synoviae MS-H vaccine strain and the versatility, and has the advantages of high accuracy, high specificity, high sensitivity, easy operation, and rapid and reliable results.

Description

Technical field [0001] The invention belongs to the technical field of biological detection, and relates to a primer set and kit for detecting Mycoplasma MS, in particular to a dual PCR primer set and kit for simultaneously detecting MS-H vaccine strain and versatility. Background technique [0002] Avian synovial mycoplasmosis is an acute or chronic infectious disease of poultry caused by MycopLasma snoviae (MS). It is mainly characterized by respiratory type and joint disease, presenting as joint disease type synovial bursitis And tenosynovitis and subclinical respiratory diseases. Sick chickens and recessively infected chickens are the source of infection of this disease. The disease can not only spread horizontally through contact with droplets and dust from coughing and sneezing of sick chickens, but also vertically through eggs. After being infected with this disease, young chickens grow poorly, adult chickens have decreased egg production, air sacculitis reduces the quali...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/686C12Q1/04C12N15/11C12R1/35
CPCC12Q1/686C12Q1/689C12Q2600/16C12Q2537/143C12Q2565/125
Inventor 李贵阳李玉燕沈巍唐志鹏曲心怡巩新民王熊祝永华沈政郭龙宗张霞
Owner 青岛英赛特生物科技有限公司
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