Oral nanoparticles of artemisinin-loaded citrus pectin
A technology of nanoparticles and artemisinin, which is applied in the field of oral nanoparticles of citrus pectin, can solve the problems of good biocompatibility, strong antigenicity, and not found, and achieve obvious sensitivity, enhanced stability, and high loading The effect of drug efficiency
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0105] Example 1 Preparation of Oral Nanosystem Loaded with Artemisinin & Citrus Pectin
[0106] The preparation process of the oral nano-examples loaded with artemisinin / citrus pectin is as follows: figure 1 shown:
[0107] Step 1: Preparation of MCP(ART) Nanoparticles
[0108] First, denature the citrus pectin, put the citrus pectin in distilled water at a concentration of 1.5 mol / L, and increase its pH to 10.0 with sodium hydroxide (3 mol / L) at 55 °C, and continue stirring for 1 hour , then the solution was cooled to room temperature, the pH was adjusted to 10.0 with sodium hydroxide (3 mol / L), and the denatured citrus pectin was dissolved.
[0109] Then add the artemisinin dissolved in the organic solvent under the condition of 55 ℃, make the final concentration of artemisinin be 1.5mol / L 600rmp stir 2 hours, add calcium hydroxide to its final concentration is 0.01mol / L, Stir at 600 rmp at 55°C for 1 hour, finally add sodium bicarbonate to a final concentration of 0.01 ...
Embodiment 2
[0115] Example 2 Characterization of Oral Nanoparticles Loaded with Artemisinin & Citrus Pectin
[0116] 1. Infrared spectroscopy detection
[0117] The raw materials MCP, AAPBA, TMC synthesized by the system and the synthetic materials MCP(ART), MCP(ART)-AAPBA and MCP(ART)-AAPBA-TMC prepared in Example 1 were used for infrared spectrum detection.
[0118] 1. Experimental method
[0119] Freeze-dry the sample to be tested, then put it in a mortar, add a certain amount of KBr, grind the mixture evenly, and grind the mixture to a particle size of less than 2 μm to avoid the influence of scattered light, and then put it in a dryer for drying. The mixture was pressed into a transparent sheet on a hydraulic press with a pressure of about 10 MPa, and was measured using a German Bruker VERTEX 33 Fourier transform infrared spectrometer.
[0120] 2. Experimental results
[0121] In order to understand the changes in the formation stage of artemisinin-loaded / citrus pectin particles, ...
Embodiment 3
[0165] Example 3 Cell experiment of oral nanoparticles loaded with artemisinin & citrus pectin
[0166] 1. Experimental method
[0167] The biotoxicity of MCP(ART)-AAPBA-TMC oral nanotransporters at the cellular level will be evaluated by MTT assay.
[0168] 1. Cell culture
[0169] HepG-2 cell line was provided by Guangdong University of Pharmacy. After the cells were proliferated and cultured to 80% in the culture flask, they were seeded on a 96-well plate at a density of 5000 / well and cultured for 1 or 2 days for subsequent experiments. The cell culture conditions are: high glucose DMEM medium containing 20% newborn bovine serum and non-essential amino acids, 37°C, 5.0% CO 2 .
[0170] 2. MTT transport system toxicity
[0171]Different doses of the transport system were added to the cultured cells, and after 24 hours, the cell viability was measured by MTT method. That is, inoculate HepG-2 cells on a 96-well culture plate, 5000 cells / well, after culturing for 24 hou...
PUM
Property | Measurement | Unit |
---|---|---|
particle size | aaaaa | aaaaa |
encapsulation rate | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com