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Application of total polysaccharides of Radix Platycodonis in preparation of drugs to treat CCCP-induced (carbonyl cyanide 3-chlorophenylhydrazone induced) apoptosis

A technology of total polysaccharide and platycodon grandiflorum, which is applied in the application field of medicine, can solve the problems such as apoptosis of platycodon grandiflorum total polysaccharide which has not been seen, and achieve the effects of relieving the phenomenon of nuclear damage, inhibiting the change of potential, and expanding the medical use.

Active Publication Date: 2019-09-06
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the anti-apoptosis induced by CCCP.

Method used

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  • Application of total polysaccharides of Radix Platycodonis in preparation of drugs to treat CCCP-induced (carbonyl cyanide 3-chlorophenylhydrazone induced) apoptosis
  • Application of total polysaccharides of Radix Platycodonis in preparation of drugs to treat CCCP-induced (carbonyl cyanide 3-chlorophenylhydrazone induced) apoptosis
  • Application of total polysaccharides of Radix Platycodonis in preparation of drugs to treat CCCP-induced (carbonyl cyanide 3-chlorophenylhydrazone induced) apoptosis

Examples

Experimental program
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Embodiment 1

[0038] Example 1: Preparation and structural analysis of Platycodon grandiflorum total polysaccharides

[0039] 1. Preparation of Platycodon grandiflorum total polysaccharide:

[0040] Cut platycodon root into slices, add 8 times the weight of water to soak overnight, then extract at 80°C for 4 hours, filter, heat and concentrate the filtrate to a relative density of 1.05-1.15 (measured at 50°C), and centrifuge at 3000rpm for 10min after cooling to remove Impurities, to obtain the extract;

[0041] Slowly add ethanol with a volume concentration of 95% to the extract, and stir while adding, so that the final concentration of ethanol is 80%, let it stand for 24 hours, centrifuge, separate the precipitate, and dry the precipitate in vacuum at 60°C to obtain Platycodon grandiflorum Total polysaccharides (PGPS t ).

[0042] 2. Structural analysis of Platycodon grandiflorum polysaccharides:

[0043] Carry out infrared spectroscopic analysis, molecular weight analysis, monosaccha...

Embodiment 2

[0070] Example 2: Study on the protective effect of Platycodon grandiflorum total polysaccharides on CCCP-induced apoptosis of porcine alveolar macrophages

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Abstract

The invention discloses application of total polysaccharides of Radix Platycodonis in the preparation of drugs to treat CCCP-induced (carbonyl cyanide 3-chlorophenylhydrazone induced) apoptosis. A mitochondrial pathway-induced apoptosis model is established with CCCP to study influence of the total polysaccharides of Radix Platycodonis upon cell activity and apoptosis after CCCP-induced oxidativeinjury occurs to porcine alveolar macrophages. It is discovered herein for the first time that the total polysaccharides of Radix Platycodonis having a certain concentration (100-200 mu g / mL) can antagonize CCCP-induced apoptosis, nuclear deformation is relieved evidently, and change in the potential of mitochondrial membrane can be inhibited. The pharmaceutical uses of the total polysaccharides of Radix Platycodonis are expanded herein; the development of novel indication drugs using total polysaccharides of Radix Platycodonis as effective ingredients is benefited.

Description

technical field [0001] The invention relates to the field of medical applications of platycodon grandiflora total polysaccharides, in particular to the application of platycodon grandiflorum total polysaccharides in the preparation of medicines for treating apoptosis induced by CCCP. Background technique [0002] Carbonyl cyanide m-chlorophenylhydrazone (CCCP) is an uncoupling agent that can inhibit mitochondrial oxidative phosphorylation, increase intracellular oxygen consumption, keep cells in a hypoxic state, and destroy the biological oxidation and phosphorylation phase. coupling effect. In the case of normal electron transport chain process, CCCP inhibits the phosphorylation process from ADP to ATP by eliminating the proton concentration gradient across the mitochondrial transmembrane, which affects the protein synthesis in the mitochondria, resulting in step-by-step cell damage until cell apoptosis. Death. [0003] Campanulaceae (Platycodon grandiflorum (Jacq) A.DC) ...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61K36/34A61P35/00C08B37/00
CPCA61K31/715A61K36/34A61P35/00C08B37/0003C08B37/006A61K2236/331A61K2236/333A61K2236/39
Inventor 赵晓娜刘建柱王成程国栋
Owner SHANDONG AGRICULTURAL UNIVERSITY
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