Primer probe combination for guiding detection of fluvastatin drug individualized drug use related gene, kit and application of kit

A fluvastatin, primer and probe technology, applied in the field of in vitro diagnostics, can solve the problems of lack of kit products, adverse reactions of clinical medication, etc., and achieve the effects of accurate and reliable detection results, low cost, and good practicability

Inactive Publication Date: 2019-09-06
南京派森诺基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to solve the technical problems of traditional clinical experience medical diagnosis and fluvastatin drug administration, which are prone to clinical adverse reactions and there is no suitable kit product

Method used

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  • Primer probe combination for guiding detection of fluvastatin drug individualized drug use related gene, kit and application of kit
  • Primer probe combination for guiding detection of fluvastatin drug individualized drug use related gene, kit and application of kit
  • Primer probe combination for guiding detection of fluvastatin drug individualized drug use related gene, kit and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] 1. Biological samples: The biological materials used in the present invention are all from within the company. Anticoagulant for the remaining 100 cases tested in the company during September-December 2018.

[0064] 2. Take the DNA extracted from the sample to be tested as a PCR template: the DNA extraction kit is the extraction kit "Blood Genomic DNA Extraction Kit" from Tiangen Biochemical Technology (Beijing) Co., Ltd. (article number: DP318).

[0065] 1. Take 400ul of whole blood, add 800ul of cell lysate CL, mix well, centrifuge at 10000rpm / 11500×g for 1min, discard the supernatant, and repeat once if the lysis is not complete.

[0066] 2. Add 200ul buffer GS to the precipitation, mix well, then add 20ul proteinase K, mix well.

[0067] 3. Add 200ul buffer GB, mix well, 56°C, 10min, invert several times during the period, until the solution becomes clear (if not clear, you can extend the time until clear).

[0068] 4. Add 200ul absolute ethanol, mix well, and cen...

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Abstract

The invention discloses a specific primer probe combination for guiding a fluvastatin drug individualized drug use related gene SNP, and is characterized in that the primer probe combination includesa specific primer probe combination for rs4783961, rs11045819 and rs1922242 genes SNP. The invention also provides a kit containing the primer combination and an application thereof. The beneficial effects comprise that provided are the quick typing kit for guiding the fluvastatin drug individualized drug use related gene SNP and the application thereof. The test result obtained by the kit is accurate and reliable, the cost is low, and the kit has good practicability.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a combination of primers and probes, a kit and an application for guiding the detection of genes related to individualized fluvastatin medication. Background technique [0002] Fluvastatin (fluvastatin) is an excellent hypolipidemic drug, and it is the first fully chemically synthesized cholesterol-lowering drug. Converted to 3-methyl-3,5-dihydroxyvaleric acid. If patients take a large amount of statins, they are prone to adverse reactions such as liver damage, muscle toxicity, and renal dysfunction, and the incidence rate will increase exponentially. [0003] "Technical Guidelines for the Detection of Drug Metabolizing Enzymes and Drug Target Genes (Trial)" points out that the detection of drug metabolizing enzymes and drug target genes can guide clinical selection of appropriate drugs and dosages for specific patients to achieve "individualization". "Medicine, ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6883C12Q1/6858C12Q2600/106C12Q2600/156C12Q2531/113C12Q2561/101
Inventor 刘颖宣涛杜晴晴赵艳伟孙子奎
Owner 南京派森诺基因科技有限公司
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