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Molecular marker, primers and detecting method for detecting maize aphid resistance gene and application

A molecular marker and resistance gene technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. Effect

Active Publication Date: 2019-09-10
HENAN AGRICULTURAL UNIVERSITY
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  • Description
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Problems solved by technology

[0004] At present, the prevention and control of aphids mainly include: clearing the weeds in the field, reducing the source of early insects, applying granules before the aphids bloom at the heart leaf stage of corn, and spraying control at the seedling stage and early tasseling stage. The above methods are all after corn planting Therefore, it is particularly important to develop a gene that can detect aphid resistance in maize and use it to breed aphid-resistant maize varieties.

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  • Molecular marker, primers and detecting method for detecting maize aphid resistance gene and application
  • Molecular marker, primers and detecting method for detecting maize aphid resistance gene and application
  • Molecular marker, primers and detecting method for detecting maize aphid resistance gene and application

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Embodiment Construction

[0025] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments, but should not be construed as a limitation of the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples can be obtained from commercial sources unless otherwise specified.

[0026] The present invention provides a molecular marker for detecting maize aphid resistance gene, and a molecular marker for detecting maize aphid resistance gene, characterized in that the molecular marker is FM.rm1, and the nucleotide sequence is as shown in SEQ ID NO.1 shown.

[0027] The above molecular marker FM.rm1 can be obtained as follows:

[0028] Using genome-wide association analysis (eg figure 1 As shown), a resistance gene was identified, and according to the difference of the resistance ...

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Abstract

The invention belongs to the technical field of maize gene detection, and particularly relates to a molecular marker, primers and detecting method for detecting a maize aphid resistance gene and application. The molecular marker is a molecular marker FM.rm1 and has the nucleotide sequence shown as SEQ ID NO.1. The upstream primer FM.rm1-F has the sequence of 5'-AAAGCAAGGCAGCAAAAGC-3', and the downstream primer FM.rm1-R has the sequence of 5'-TGCGTGATGCAGTTGAGCA-3'. The molecular marker can detect the maize aphid resistance gene, is high in detection efficiency and accuracy and has good application prospects in the cultivation of the aphid-resistant maize species.

Description

technical field [0001] The invention belongs to the technical field of maize gene detection, in particular to a molecular marker, primer, detection method and application for detecting maize aphid resistance genes. Background technique [0002] Corn aphid (Rhopalosiphum maidis) is one of the harmful pests in the growth process of corn. It mainly feeds on the back of leaves, heart leaves, filaments and tassel, and can secrete "honeydew" and often in the injured part. Black mildew is formed, which affects photosynthesis, and the edges of leaves turn yellow; if it occurs on the tassel, it will affect pollination and lead to reduced yield; the severely damaged plants have thin ears, not full grains, and long bald tips. In addition, aphids can transmit maize dwarf mosaic virus and red leaf virus, causing viral diseases that cause greater yield losses. [0003] There are about 20 generations a year, and the adult and nymph aphids overwinter in the hearts and leaves of the grasses...

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 陈甲法吴建宇韩春燕杨宁宁
Owner HENAN AGRICULTURAL UNIVERSITY