Method for Visually Determining Hydrogen Peroxide Content Using Doped Carbon Quantum Dots

A technology of carbon quantum dots and hydrogen peroxide, which is applied in the fields of molecular detection and medicinal chemistry, can solve the problems of low sensitivity detection limit, complex detection process, time-consuming, high cost, etc., and achieve good selectivity, simple detection process, and low detection limit Effect

Active Publication Date: 2021-07-20
NORTHWEST NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for visually detecting hydrogen peroxide using a boron nitrogen sulfur doped carbon quantum dot smart phone. The method has high sensitivity, low detection limit, good selectivity, simple detection process, and overcomes the conventional spectrum, The shortcomings of chromatographic detection process are complicated, time-consuming and high cost

Method used

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  • Method for Visually Determining Hydrogen Peroxide Content Using Doped Carbon Quantum Dots
  • Method for Visually Determining Hydrogen Peroxide Content Using Doped Carbon Quantum Dots
  • Method for Visually Determining Hydrogen Peroxide Content Using Doped Carbon Quantum Dots

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Effect test

Embodiment 1

[0029] The synthesis of embodiment 1, BNS-CDs

[0030] Weigh 0.075g of 2,5-diaminobenzenesulfonic acid and 0.075g of 4-aminophenyl borate hydrochloride respectively in a 100mL beaker, add 30mL of deionized water, ultrasonicate for 5 minutes to fully dissolve, and then transfer the mixed solution Put it into a 50mL polytetrafluoroethylene high-pressure reactor, and conduct a hydrothermal reaction at 180°C for 8 hours. After the reaction, cool to room temperature to obtain a purple-red solution, centrifuge at 1000r / min for 15 minutes, take out the supernatant and filter it with a 0.22um filter membrane. Then use a dialysis bag with a molecular weight cut-off of 1000Da to dialyze for 24 hours, remove a large amount of solvent by rotary evaporation of the ambient solution outside the bag, and finally freeze-dry to obtain a red powder and store it at 4°C.

Embodiment 2

[0031] The mensuration of embodiment 2, Pien Tze Huang mouthwash

[0032] Add 0.3mL of 100µg / LBNS-CDs solution, 0.25mL of 6mMTB solution, 1.5mL of Pien Tze Huang mouthwash and pH=3.00 acetic acid-acetate buffer solution to a 10mL glass centrifuge tube in turn, dilute the total volume to 3mL, and dilute the above mixed solution After oscillating evenly, react at 35°C for 80 minutes. After the reaction, take out the centrifuge tube and insert it into the sample port of the mobile phone test box (refer to CN201810932563.2 for its structure). Use the smartphone to obtain the RGB value of the color intensity of the solution, and select based on the R channel The data model for the color intensity response value, by Y R =|I R -I R blank |The formula calculates the color intensity response value of the solution, and Y R Substituting the value into the linear regression equation of hydrogen peroxide, the content of hydrogen peroxide in Pien Tze Huang mouthwash is calculated to be 1...

Embodiment 3

[0033] Embodiment 3, the mensuration of Korean mouthwash

[0034] Add 0.3mL of 100µg / LBNS-CDs solution, 0.25mL of 6mMTMB solution, 1.0mL of Pien Tze Huang mouthwash and pH=3.00 acetic acid-acetate buffer solution to a 10mL glass centrifuge tube in order to make the total reaction volume 3mL, shake the above mixed solution After uniformity, react at 35°C for 80 minutes. After the reaction, take out the centrifuge tube and insert it into the sample inlet of the mobile phone test box, use the smartphone to obtain the color intensity RGB value of the solution, and select the data model based on the R channel as the color intensity response value. press Y R =|I R -I R blank |The formula calculates the color intensity response value of the solution, and Y R Substituting the value into the linear regression equation of hydrogen peroxide, it is calculated that the content of hydrogen peroxide in Pien Tze Huang mouthwash is 2.93+0.33µM.

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Abstract

The invention discloses a method for visually measuring the content of hydrogen peroxide using a carbon quantum dot smart phone. Carbon quantum dots BNS-CDs doped with water-soluble boron, nitrogen and sulfur three elements are used as hydrogen peroxide catalytic enzymes. In the acetate buffer solution with 3',5,5'-tetramethylbenzidine as the substrate, hydrogen peroxide is catalyzed to produce hydroxyl radicals (∙OH) to promote the oxidation of TMB to produce blue oxides, which are then used in smartphones The color-picking software Color Grab obtains the RGB value of the blue oxide generated by the oxidation of TMB to quantitatively determine hydrogen peroxide. The method has high sensitivity, low detection limit, good selectivity, simple detection process, and can realize the visual and rapid detection of trace hydrogen peroxide.

Description

technical field [0001] The invention relates to a method for detecting the content of hydrogen peroxide, in particular to a method for visually measuring the content of hydrogen peroxide by using a carbon quantum dot mobile phone, and belongs to the technical field of molecular detection and the technical field of medicinal chemistry. Background technique [0002] As a commonly used disinfectant, hydrogen peroxide is widely used in the food industry. However, active or passive excessive intake of hydrogen peroxide will cause certain harm to the human body, for example, it can cause human genetic material DNA damage and gene mutation, accelerate the aging process of the human body, and be associated with cancer, diabetes, cardiovascular disease, and neurological atrophy. disease related. Therefore, it is necessary to develop an effective method for quantitative determination of hydrogen peroxide concentration. At present, some common methods such as titration, chromatograph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/35
CPCG01N21/35G01N2021/3595
Inventor 彭波徐佳美樊祢祢郭阳房延军
Owner NORTHWEST NORMAL UNIVERSITY
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