Rapid three-dimensional imaging method applicable to optical transparent sample

A three-dimensional imaging and sample technology, applied in the field of image processing technology and microscopic imaging, can solve the problems of unsatisfactory resolution, difficult adjustment, low numerical aperture NA value, etc.

Active Publication Date: 2019-09-17
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology

The current imaging method suitable for optically transparent samples is mainly light sheet microscopy. However, this method relies on two mutually orthogonal optical paths to excite and detect the sample. The optical paths are ver

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  • Rapid three-dimensional imaging method applicable to optical transparent sample
  • Rapid three-dimensional imaging method applicable to optical transparent sample

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Embodiment Construction

[0017] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the associated drawings. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0018] In this example, the mouse brain sample is used as the research object to explain the specific implementation process of the present invention. The thickness of the mouse brain sample is 1.5 mm. When directly using an optical microscope for three-dimensional detection, the imaging depth is limited to about 10 μm due to the influence of light scattering. . In order to improve the imaging depth of the optical microscope, the mouse brain samples nee...

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Abstract

The invention provides a rapid three-dimensional imaging method applicable to an optical transparent sample. The method comprises the steps of S1, training a convolution neural network; S2, processing a sample, in which a to-be-imaged sample is fixed after optical transparent processing; S3, acquiring an optical chromatography image of a shallow, in which wide-field images of the shallow layer are acquired and are input to the convolution neural network of which training is completed, and optical chromatography images which are same in numbers and sizes of the wide-field images are obtained; and S4, acquiring the optical chromatography image of the whole optical transparent sample. According to the method, a three-dimensional image of the sample is obtained by high-flux wide-field imaging and rapid axial scanning, and finally, a clear and complete three-dimensional image is restored by the convolution neural network; and by the method, the image shooting speed is remarkably increased, and a photobleaching effect is reduced.

Description

technical field [0001] The invention relates to image processing technology and microscopic imaging technology, in particular to a fast three-dimensional imaging method suitable for light-transparent samples. Background technique [0002] When using an optical microscope to detect thick tissue samples, the deep tissue will seriously absorb and scatter the incident light, resulting in limited imaging depth. Using the light-transparency method can make the tissue exhibit better light-transparent characteristics, and light incident into the tissue will not undergo large absorption or scattering. The current imaging method suitable for light-transparent samples is mainly light-sheet microscopy. However, this method relies on two mutually orthogonal optical paths to excite and detect the sample, and the optical paths are very complicated and difficult to adjust. In addition, most of the objective lenses used in light sheet microscopes are air mirrors with long working distances,...

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Application Information

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IPC IPC(8): G01N21/84G01N1/28G06N3/04G06T11/00
CPCG01N21/84G01N1/28G06T11/003G06N3/045
Inventor 龚辉袁菁宁可夫张小宇
Owner HUAZHONG UNIV OF SCI & TECH
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