12 results about "Wide field imaging" patented technology

The invention provides a light source multiplexing diamond NV color center wide field detection system and an illumination detection method. The system comprises a diamond containing an NV color center, an exciting light module, a first double-color sheet, an objective lens, a second double-color sheet, a wide field imaging module, an illumination imaging module and a microwave module, exciting light generated by the exciting light module irradiates the first double-color piece, is guided to the objective lens, and irradiates the diamond after being transmitted by the objective lens so as to excite the NV color center of the diamond to generate fluorescent light; the objective lens is of an infinity type; the exciting light is irradiated to a sample to be detected after being transmitted by the diamond; fluorescent light and exciting light from a sample to be detected are collected by the objective lens, then transmitted to the first double-color piece and guided to the second double-color piece through the first double-color piece, the second double-color piece guides the fluorescent light to the wide-field imaging module for imaging, and the exciting light in the second double-color piece is guided to the illumination imaging module for imaging. Illumination can be achieved in the exciting light working state, exciting light switching operation in the prior art is avoided, and efficiency can be remarkably improved.

The application discloses a kind of multi-mode optical fiber wavelength multiplexing wide field imaging quality promotion methods based on dynamic calibration.The method comprises the following steps: collecting the output speckle pattern corresponding to each wavelength after being transmitted by multi-mode optical fiber;The speckle patterns of different wavelengths are spatially registered and spliced to form a high-dimensional composite speckle vector;PCA is used to bidirectionally compress the dimension of input image and composite speckle vector group, and a linear wavelength multiplexing inverse transmission matrix (WITM) is dynamically estimated and updated in low-dimensional space, and real-time pre-reconstruction and continuous monitoring are realized;Set up optical fiber state monitoring module to analyze pre-reconstruction frame in real time, when structural disturbance or optical fiber deformation is detected, automatically trigger in situ dynamic calibration, realize the rapid adaptive update of WITM, without retraining network.The updated WITM will guide the diffusion neural network image optimizer to iteratively denoise and detail repair the pre-reconstruction result, significantly improve the image fidelity and structure restoration ability.

The utility model provides a multi-mode fluorescence microscope. The multi-mode fluorescence microscope comprises a microscopic assembly, a near-infrared two-region excitation light path, a visible light confocal excitation light path, a wide-field imaging light path, a near-infrared two-region confocal imaging assembly, a multi-photon intensity / life harmonic wave imaging assembly and a visible light confocal and wide-field imaging assembly, the microscopic assembly comprises an objective lens, an optical filter switching assembly, a sleeve lens and a transflective device which are sequentially arranged from the object side to the image side, and the transflective device can be switched to a dichroscope or a reflector; the near-infrared second-area excitation light path, the visible light confocal excitation light path and the wide-field imaging light path are connected with the sleeve lens; the near-infrared two-region confocal imaging assembly, the multi-photon intensity / lifetime and harmonic imaging assembly and the visible light confocal and wide-field imaging assembly are all connected to the sleeve lens. According to the scheme, confocal and wide-field imaging of visible light, confocal and wide-field imaging of a near-infrared second region, and multi-photon intensity / lifetime and harmonic imaging based on near-infrared excitation are realized.

The invention provides an infinite far field microscope objective and a quantum wide field imaging device, and the first lens group comprises a plurality of lenses and has positive focal power; the second lens group comprises a first lens with positive focal power, a first balsaming lens with negative focal power, a second balsaming lens with negative focal power, a seventh lens with positive focal power and a diaphragm which are sequentially arranged from the object side to the image side; or the first lens with the positive focal power, the first bonding lens with the negative focal power, the second bonding lens with the positive focal power, the seventh lens with the negative focal power and the diaphragm are sequentially arranged from the object side to the image side. The red fluorescence wave band of the diamond NV color center can be well matched, and the dual requirements of the large numerical aperture and the working distance can be met.

The invention discloses a multimode fiber wavelength multiplexing wide-field imaging quality improving method based on dynamic calibration. The method comprises the following steps: acquiring output speckle patterns corresponding to wavelengths after multimode optical fiber transmission; performing spatial registration and splicing on the speckle patterns with different wavelengths to form a high-dimensional composite speckle vector; performing bidirectional compression dimension reduction on an input image and a composite speckle vector group by adopting PCA (Principal Component Analysis), dynamically estimating and updating a linear wavelength multiplexing inverse transmission matrix (WITM) in a low-dimensional space, and performing real-time pre-reconstruction and continuous monitoring; an optical fiber state monitoring module is arranged to analyze a pre-reconstruction frame in real time, when structural disturbance or optical fiber deformation is detected, in-situ dynamic calibration is automatically triggered, rapid adaptive updating of the WITM is achieved, and the network does not need to be trained again. And the updated WITM guides a diffusion type neural network image optimizer to carry out iterative denoising and detail restoration on a pre-reconstruction result, so that the image fidelity and the structure restoration capability are remarkably improved.

The invention provides an integrated detection system based on a solid-state spinning color center and a nondestructive detection method. The integrated detection system comprises an excitation light module, a wide-field branch, a confocal branch, a first beam splitter, a first double-color sheet, an objective lens, a probe comprising a solid-state spin color center, a first filter, a guide element, a first photoelectric detection light path, a second photoelectric detection light path, a wide-field imaging light path, a differential module and a microwave module. The excitation light module outputs two paths of excitation light in a switchable manner, the two paths of excitation light are respectively transmitted to a confocal branch and a wide-field branch, the two paths of light beams are guided to the same laser excitation and fluorescence detection light path through a first beam splitter, and then collected fluorescence is guided to a first photoelectric detection light path or a wide-field imaging light path through a guide element; meanwhile, after the exciting light output by the confocal branch is split by the first beam splitter, one part of the exciting light is used for excitation, and the other part of the exciting light is used for difference, so that common-mode noise can be reduced; wide-field imaging and confocal integration can realize real-time switching.

The invention discloses a stable multimode optical fiber wide-field imaging method. The method comprises the following steps: establishing a multimode fiber wide-field imaging system based on dual-wavelength laser; collecting image information and optical fiber form information respectively carried by a large number of lasers with different wavelengths in the optical fiber form; constructing an optical fiber form recognition model and an image reconstruction model library adapted to different optical fiber forms; the optical fiber form is identified in real time, and an image reconstruction model is selected from a database for imaging. Compared with an existing method, the method is beneficial for reducing the influence of optical fiber form change and improving the imaging stability of the multimode optical fiber wide-field imaging system.

The application discloses a kind of annular focusing scanning illumination's reflection wide field super-resolution optical microscopic system and method, system includes sample displacement module, annular light beam generation module, light beam scanning module, super-resolution focusing illumination and collection module, optical microscopic imaging module, phase contrast module and computer.By utilizing annular light beam illumination, the advantage of high focusing efficiency of conventional lens, fast light beam scanning speed, realize fast, efficient super-resolution focusing illumination, and then can be realized fast super-resolution optical microscopic imaging using conventional optical lens;By two-dimensional light beam scanning illumination, realize non-labeled far-field super-resolution two-dimensional microscopic fast wide field imaging;By the non-labeled far-field super-resolution two-dimensional microscopic wide field imaging of sample at different axial positions, obtain the super-resolution two-dimensional microscopic wide field imaging of different cross sections of sample, and then realize three-dimensional tomography.The application can be applied to non-labeled super-resolution microscopic fast imaging of biological sample, and can also be applied to industrial related super-resolution microscopic detection and other fields.

The invention provides a diamond NV color center wide field detection system capable of continuously illuminating and an illumination detection method. The system comprises a diamond containing an NV color center, an excitation light module, a first double-color sheet, an objective lens, a second double-color sheet, a wide field imaging module, an illumination light module, an illumination imaging module and a microwave module, exciting light generated by the exciting light module irradiates to the first double-color piece, is guided to the objective lens and irradiates to the diamond after being transmitted by the objective lens, and illumination light generated by the illumination light module irradiates to a sample to be detected; the wavelength of the illumination light is different from that of the fluorescence; fluorescent light generated by excitation of the diamond and illumination light from a to-be-detected sample are collected by the objective lens, then transmitted to the first double-color piece and guided to the second double-color piece through the first double-color piece, the second double-color piece guides the fluorescent light in the second double-color piece to the wide-field imaging module for imaging, and the illumination light is guided to the illumination imaging module for imaging. Therefore, continuous illumination detection during wide-field imaging work is realized, and the efficiency is improved.

The present application relates to the technical fields of laser wide field imaging sensor, and discloses a temperature sensing wide field probe, which comprises a plurality of detection units and a substrate, wherein the substrate is a poor conductor of heat, there is a heat insulation spacing between adjacent detection units, and the detection units are diamond particles containing NV color centers; the diamond particles are designed to be spaced apart on the substrate with low thermal conductivity to form a wide field probe, which can effectively weaken the mutual interference of the surrounding temperature during temperature detection, thereby ensuring the accuracy and high sensitivity of wide field temperature measurement; the chip temperature measurement system provided in the present application is directly irradiated on the temperature sensing wide field probe by a diffuse trigger light, the structure is simple and easy to realize, and the number of NV color center particles is less limited, and a large number of spaced NV color center particles can achieve high sensitivity detection results.

The application discloses a kind of based on deep learning's structured light super-resolution microscopic imaging method and system.The system includes light source, first lens, polarized light splitting prism, spatial light modulator SLM, second lens, photographic mode switching module, third lens, microscopic objective, three-dimensional motorized stage, dichroic mirror, barrel lens, color filter and camera in turn along the direction of light path;The photographic mode switching module includes three modes: 3D-SIM mode, 2D-SIM mode and wide field mode.The method is a kind of based on deep learning's, key frame auxiliary under structured light illumination microscopic super-resolution imaging method.The application combines wide field imaging with structured light illumination super-resolution microscopic imaging technology, can reduce the phototoxicity and photobleaching in the process of structured light illumination super-resolution microscopic imaging, realize long time super-resolution dynamic observation to submicrostructure of living cell.

The application belongs to the technical field of full-eye imaging medical equipment, and particularly relates to a handheld full-eye OCT device for realizing wide-area imaging, which comprises an imaging system, a control system, a reference arm module and a sample arm module; the sample arm module comprises a galvanometer module and a switching module arranged in sequence on an output light path of a fiber interference module; the switching module comprises a third achromatic doublet lens group, a flip mirror structure and a fourth achromatic doublet lens group arranged in sequence on an output light path of the galvanometer module, and the third and fourth achromatic doublet lens groups form a telescope system; the switching module switches different imaging positions through the flip mirror structure. Compared with the prior art, the application solves the single imaging of the prior art which is mainly used for the anterior segment or posterior segment of the eye, and the field of view is small, it is difficult to fully cover the entire posterior segment of the eye, and the traditional fundus photography technology also has the problem of limited field of view; the scheme can realize wide-angle imaging of a large field of view in the posterior segment of the eye while switching the imaging positions of the anterior segment and the posterior segment.