Fluorescence control method used in labeling biological tissues with organic fluorescent dye molecules

A technology of fluorescent dyes and control methods, which is applied in the field of fluorescence control of organic fluorescent dye molecularly labeled biological tissues, can solve the problems of reducing the conjugated π electron density of organic fluorescent molecules, the difficulty of accurate three-dimensional registration, and the interference of imaging background fluorescence, etc., to achieve Suppression of interference from background fluorescence, reversible quenching, and convenient reactivation
CN106568753AActive Publication Date: 2017-04-19HUAZHONG UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAZHONG UNIV OF SCI & TECH
Publication Date
2017-04-19

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Abstract

The invention discloses a fluorescence control method used in labeling biological tissues with organic fluorescent dye molecules. According to the fluorescence control method, hexatomic rings are formed via combination of metal ions with the organic fluorescent dye molecules, and quenching of the fluorescence of the organic fluorescent dye molecules is realized; coordination bonds are formed by a metal ion chelating agent with metal ions, combination of the metal ions with the organic fluorescent dye molecules is destroyed, and reactivation of the fluorescence of the organic fluorescent dye molecules is realized. The fluorescence control method is capable of realizing complete fluorescence quenching, and convenient and controllable reactivation. The fluorescence control method used in labeling biological tissues with organic fluorescent dye molecules is combined with a wide-field imaging system, so that chemical chromatography imaging of samples labeled with the fluorescent dye molecules is realized, and rapid chemical chromatography imaging of large-size biological tissues labeled with organic fluorescent molecules is realized.
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Description

technical field

[0001] The invention belongs to the field of biological imaging, and more specifically relates to a fluorescence control method for molecularly marking biological tissues with organic fluorescent dyes. Background technique

[0002] Obtaining the detailed spatial distribution of molecules and proteins in biological tissues is an important basis for understanding the relationship between the structure and function of organisms. Fluorescent probe labeling technology can label specific molecules and proteins. In order to better study the spatial distribution and function of these molecules and proteins in biological tissues, a variety of fluorescent probe labeling methods in the same organism have been developed. It is widely used in the fields of biology and medicine.

[0003] For optical imaging of biological tissues labeled with fluorescent probes, there are mainly two existing physical tomography techniques: one is optical tomography, which optically suppres...

Claims

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