Immune cell cryopreservation liquid and cryopreservation method

Abstract

The invention discloses an immune cell cryopreservation liquid and a cryopreservation method. A cryopreservation device comprises a heat preservation box, a temperature control cavity with an upward opening is arranged in the heat preservation box, a sealing plate is fixedly connected to the upper end of the temperature control cavity; and four injection devices are arranged in an annular array on the sealing plate. A mechanical claw type clamping switch structure is used to avoid direct contact between an operator and a cell test tube, and ensures that cells are not polluted, and an integrated sealed sterile temperature control barrel structure is simultaneously adopted to avoid contact of the cells with the outside air in the treatment process before cell freezing, ensure that the cells may not denature, complete the processes of centrifugation and addition of a freezing liquid in the natural falling process of a cell liquid from top to bottom, and reduce the time required for freezing, and regionalization temperature drops from top to bottom, so that water in the cells may not generate ice crystals first due to the temperature to affect the own activity of the cells.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to an immune cell cryopreservation solution and a cryopreservation method. Background technique [0002] The cryopreservation process of cells will significantly change the thermodynamic, chemical and physical environment of pluripotent stem cells, causing damage to cells, and improper cryopreservation may even lead to cell death. The traditional cryopreservation process needs to be divided into steps. First, the cells are placed in 1000rpm for five minutes, then the supernatant is screened, and then the cell cryopreservation solution is added, filtered in a freezer at -80 degrees, and finally moved to liquid nitrogen for storage. . This process will design the direct contact between the operator and the reagent tube for many times, which has been exposed to the external environment, so that the cells will be polluted and affect the subsequent research or treatment effect. The...

AI Technical Summary

Problems solved by technology

This process will design the direct contact between the operator and the reagent tube for many times, which has been exposed to the external environment, so that the cells will be polluted and affect the subsequent research or treatment effect. A device that can solve the above problems is explained in the present invention.

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