Polyethylene-degradable Pichia guilliermondii

A technology of polyethylene and yeast, applied in the direction of microorganism-based methods, microorganisms, fungi, etc., can solve the problems of air pollution, environmental pollution, etc.

Inactive Publication Date: 2019-09-20
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the waste polyethylene plastics are mainly processed by incineration and landfill, and waste plastics account for 5-25wt% of the total waste in landfills, and the landfilling of a large amount of plastic waste has caused management problems and environmental haza

Method used

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  • Polyethylene-degradable Pichia guilliermondii
  • Polyethylene-degradable Pichia guilliermondii
  • Polyethylene-degradable Pichia guilliermondii

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specific Embodiment approach 1

[0014] Specific embodiment 1: Pichia pastoris MeyerozymaguilliermondiiZJC-1, which can degrade polyethylene in this embodiment, is preserved in the General Microbiology Center (CGMCC) of China Microbial Culture Collection Management Committee, and the preservation address is No. 1, Beichen Road, Chaoyang District, Beijing Court No. 3, the deposit date is December 17, 2018, and the deposit number is CGMCCNo: 16956.

[0015] In this embodiment, Pichia guilliermondii ZJC-1 is obtained by screening the intestinal fluid of the larvae of the mellonella mellonella. Filtering is done in the following steps:

[0016] Prepare 100 larvae of the 5th instar wax moth, feed them with wheat bran as supplementary food and feed them with polyethylene film for 2-3 days, and then do fasting treatment. After 24 hours of fasting, immerse the larvae in 75% ethanol to sterilize for 1 min, and then rinse them twice with sterile saline , dissected on a clean bench to obtain intestinal fluid. Put the ...

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Abstract

The invention relates to a strain of polyethylene-degradable Pichia guilliermondii, and relates to a strain of Pichia guilliermondii. The invention provides a train of Pichia guilliermondii which can degrade polyethylene and provides a new bacterial source for the biodegradation of polyethylene. The Pichia guilliermondii is Meyerozyma guilliermondii ZJC-1, and is deposited in the China General Microbiological Culture Collection Center, with the preservation address being No.3, Courtyard 1, Beichen Road, Chaoyang District, Beijing, the preservation date being December 17, 2018, and the preservation number being CGMCC No. 16956. The Meyerozyma guilliermondii ZJC-1 provides a new bacterial source for biodegradable polyethylene, and is used to degrade polyethylene.

Description

technical field [0001] The present invention relates to a strain of Pichia mongolica. Background technique [0002] The production of polymer plastics is one of the fastest growing areas of global industry and has been in use for 100 years. Since the 1950s, plastic products have been produced and consumed on a large scale, and the global plastic production has shown an obvious exponential trend. In the fifty years since 1964, the scale of plastic production has increased by 20 times, and the global annual plastic production More than 300 million tons, the global plastic production was 311 million tons in 2014, 335 million tons in 2015, and is expected to reach about 1.8 billion tons by 2050. Polyethylene is one of the main raw materials of plastic products. It has large relative molecular weight, strong hydrophobicity and low surface energy. There are usually three types of polyethylene, namely high-density polyethylene, low-density polyethylene and linear low-density poly...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12R1/84
CPCC12N1/16C12R2001/84C12N1/165
Inventor 张杰刘长莉王滨松池明眼张国财王珊珊郝孔利董欣欣姬彦飞高超刘燕
Owner NORTHEAST FORESTRY UNIVERSITY
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