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General SSR primers for clematis plants and application of general SSR primers

A technology of clematis and clematis, which is applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc., can solve the problems of limited number of varieties and poor versatility, and achieve good versatility Effect

Pending Publication Date: 2019-09-20
LUOYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the large number of clematis species in my country and the many differences in genes among clematis populations, the number of clematis species applicable to the current SSR primers is limited and the versatility is poor.

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  • General SSR primers for clematis plants and application of general SSR primers
  • General SSR primers for clematis plants and application of general SSR primers
  • General SSR primers for clematis plants and application of general SSR primers

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Embodiment Construction

[0024] In order to enable those skilled in the art to better understand that the technical solutions of the present invention can be implemented, the present invention will be further described below in conjunction with specific embodiments and accompanying drawings. The following examples and the test methods for which specific conditions are not indicated in the above-mentioned summary of the invention are all carried out according to conventional methods and conditions in the art.

[0025] The present invention provides 10 pairs of SSR primers with polymorphism and good versatility. The nucleotide sequences of the 1-10th pair of SSR primers are shown in sequence as SEQ ID NO.1-SEQ ID NO.20. We also provided another 9 pairs of SSR primers with better polymorphism, and the nucleotide sequences of the 11th-19th pair of SSR primers are shown in SEQ ID NO.21-SEQ ID NO.38. The characteristics of 19 pairs of SSR primers are shown in Table 1.

[0026] Table 1 Characteristics of 19...

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Abstract

The invention belongs to the technical field of plant SSR primer development, and particularly relates to general SSR primers for clematis plants and application of the general SSR primers. The Nucleotide sequences of 1st to 10th pair of general SSR primers are sequentially shown as SEQ ID NO. 1-SEQ ID NO. 20. The general SSR primers are simultaneously suitable for four population materials of Clematis macrodentata, Clematis Shaanxi, Clematis taihang and Clematis macrophylla, have good universality, and can lay a foundation for genetic diversity analysis, germplasm resource protection, excellent gene positioning and genetic breeding of clematis plant in the future.

Description

technical field [0001] The invention belongs to the technical field of plant SSR primer development, and in particular relates to a general SSR primer for Clematis plants and its application. Background technique [0002] Clematis L. belongs to Ranunculaceae (Ranunculaceae), and there are more than 300 kinds of plants in total. Clematis L. plants have the functions of diuresis, pain relief, rheumatism, and ulcer tumor inhibition, and their economic value is also high. In addition, this genus has the characteristics of bright colors, rich flower shapes, and graceful posture, so it is also suitable for greening. Clematis plants not only have potential garden ornamental value and application prospects in urban gardens, but also have extremely high application value in medicine, and have broad comprehensive application development prospects. At present, domestic research on Clematis macrophylla mainly focuses on biological characteristics, chemical composition analysis, seed ge...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 周晓君张凯卢晓玉王海亮马文杰程彦伟孙姗姗韩建明
Owner LUOYANG NORMAL UNIV