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Method for rapidly detecting vitality of tobacco seeds

A tobacco seed and vigor technology, which is applied in the field of rapid detection of tobacco seed vigor, can solve the problems of not reflecting the level of seed vigor, and achieve the effect of saving detection time and ensuring accuracy

Inactive Publication Date: 2019-09-20
YUXI ZHONGYAN TOBACCO SEED +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using the conductivity method to measure the conductivity of untreated seeds, the results sometimes do not reflect the true vitality level of the seeds

Method used

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  • Method for rapidly detecting vitality of tobacco seeds
  • Method for rapidly detecting vitality of tobacco seeds
  • Method for rapidly detecting vitality of tobacco seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Test materials

[0027] A1, A2, B1, B2, C1, C2, D1, D2 altogether eight different vigor batches of tobacco seeds.

[0028] 2. Test method

[0029] 2.1 Seed germination rate after controlling deterioration: the germination rate obtained by standard germination test after controlling deterioration

[0030] (1) Treatment of deterioration control: Take the above 8 batches of tobacco seeds, wash them 3-5 times with deionized water, adjust the water content to 20%, seal them with aluminum foil bags, balance the moisture for 1 day, and store them at 45°C Water bath 24h;

[0031] (2) Standard germination test: take out the seeds treated in step 2.1(1), cultivate them at 20°C, 16h in the dark, 30°C, and 8h in the light, and count the germination rate on the 16th day; 4 repetitions.

[0032] 2.2 The relative conductivity of the deteriorated seeds: measure the conductivity after controlling the deterioration, and obtain the relative conductivity of the seeds by calculation ...

Embodiment 2

[0057] In this embodiment, the soaking time before the conductivity measurement is tested, and the time setting gradient is: 6h, 12h, 18h, 24h, 30h, 36h, 42h, 48h and 54h; the remaining steps are the same as in Example 1 to obtain the relative conductivity of deterioration The method is the same.

[0058] The relative conductivity of the control deterioration seeds under different soaking times in table 2

[0059]

[0060] Table 3 The regression equation and correlation coefficient of the relative conductivity of the control deterioration seeds and the germination rate of the control deterioration seeds under different soaking times

[0061]

[0062] Table 4 Regression equation and correlation coefficient of control deterioration relative conductivity and field emergence rate under different soaking times

[0063]

[0064] There are significant differences in the relative electrical conductivities measured after controlling the deterioration of tobacco seeds. Varieti...

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Abstract

The invention discloses a method for rapidly detecting the vitality of tobacco seeds. The method comprises the steps of: taking tobacco seeds to be detected, performing cleaning, adjusting the water content of the tobacco seeds to 15-25%, balancing the water content, and performing water bath for 24 hours at 45 DEG C to obtain seeds subjected to deterioration control treatment; taking the seeds subjected to deterioration control treatment, adding the seeds into deionized water with the water temperature of 20-25 DEG C for soaking for 6-54 h, and determining the absolute conductivity of the soaking solution; then placing the tobacco seeds in deionized water, heating the tobacco seeds to 100 DEG C for boiling for 10-20 min, taking out the tobacco seeds, cooling the tobacco seeds to a room temperature, and determining the total conductivity of the tobacco seeds; and calculating the relative conductivity of the tobacco seeds by taking the conductivity of the deionized water as a contrast conductivity to reflect the activity of the tobacco seeds. The method combines a deterioration control method and a conductivity method, so that the detection time is greatly saved, and the detection accuracy is ensured.

Description

technical field [0001] The invention relates to the technical field of seed production, in particular to a method for rapidly detecting the vigor of tobacco seeds. Background technique [0002] Tobacco seeds are the most important means of production in tobacco leaf production. The quality of seeds directly affects the emergence of tobacco in the field, the growth of later plants and the quality of tobacco leaves. Seed vigor is an important index to measure the quality of sowing. It is a comprehensive expression of the activity intensity and characteristics of seeds during germination and emergence, that is, the potential ability of seeds to germinate and emerge quickly and neatly under field production conditions, and to grow into normal plants. . Seeds with high vigor have fast emergence, uniform emergence and strong growth, which are suitable for the requirements of agricultural mechanization. Tobacco planting area is extensive in my country, identification and screenin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/06G01N1/28
CPCG01N1/28G01N27/06
Inventor 马文广关亚静张志豪郑昀晔潘威胡晋潘荣辉
Owner YUXI ZHONGYAN TOBACCO SEED
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