Method for rapidly detecting vitality of tobacco seeds
A tobacco seed and vigor technology, which is applied in the field of rapid detection of tobacco seed vigor, can solve the problems of not reflecting the level of seed vigor, and achieve the effect of saving detection time and ensuring accuracy
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Embodiment 1
[0026] 1. Test materials
[0027] A1, A2, B1, B2, C1, C2, D1, D2 altogether eight different vigor batches of tobacco seeds.
[0028] 2. Test method
[0029] 2.1 Seed germination rate after controlling deterioration: the germination rate obtained by standard germination test after controlling deterioration
[0030] (1) Treatment of deterioration control: Take the above 8 batches of tobacco seeds, wash them 3-5 times with deionized water, adjust the water content to 20%, seal them with aluminum foil bags, balance the moisture for 1 day, and store them at 45°C Water bath 24h;
[0031] (2) Standard germination test: take out the seeds treated in step 2.1(1), cultivate them at 20°C, 16h in the dark, 30°C, and 8h in the light, and count the germination rate on the 16th day; 4 repetitions.
[0032] 2.2 The relative conductivity of the deteriorated seeds: measure the conductivity after controlling the deterioration, and obtain the relative conductivity of the seeds by calculation ...
Embodiment 2
[0057] In this embodiment, the soaking time before the conductivity measurement is tested, and the time setting gradient is: 6h, 12h, 18h, 24h, 30h, 36h, 42h, 48h and 54h; the remaining steps are the same as in Example 1 to obtain the relative conductivity of deterioration The method is the same.
[0058] The relative conductivity of the control deterioration seeds under different soaking times in table 2
[0059]
[0060] Table 3 The regression equation and correlation coefficient of the relative conductivity of the control deterioration seeds and the germination rate of the control deterioration seeds under different soaking times
[0061]
[0062] Table 4 Regression equation and correlation coefficient of control deterioration relative conductivity and field emergence rate under different soaking times
[0063]
[0064] There are significant differences in the relative electrical conductivities measured after controlling the deterioration of tobacco seeds. Varieti...
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