Kit for quantifying paraquat in plasma by using liquid chromatography-tandem mass spectrometry, and preparation method thereof

Abstract

The invention provides a high-sensitivity kit for quantifying paraquat in plasma by using liquid chromatography-tandem mass spectrometry, and a preparation method thereof. The kit consists of a standard curve plasma sample with specific concentration, a quality control plasma sample, an internal standard solution and a mobile phase conditioning solution. The plasma sample is precipitated by usingacetonitrile with paraquat-d8 as an internal standard, Thermo acclaim trinity Q1 is used as a stationary phase, and an aqueous solution of acetonitrile-100 mmol / L ammonium formate and 0.4% formic acidis used as a mobile phase to perform chromatographic gradient separation, quantitative detection is performed by using a mass spectrometer, and ion selection channels are respectively m / z 93.1-171.2(paraquat) and m / z 97.1-179.2 (paraquat-d8). The kit provided by the invention has test sensitivity of 1.0 ng / ml, and various methodological indicators can meet the needs of quantitative detection ofplasma concentration of paraquat in plasma.

Description

technical field [0001] The invention relates to a kit for quantitative detection of paraquat in plasma developed based on liquid chromatography tandem mass spectrometry technology, in particular to a method for accurate quantitative detection of paraquat in plasma by using liquid chromatography mass spectrometry technology. [0002] The present invention also relates to the production method and application of the kit of the present invention. Background technique [0003] Paraquat (PQ) is a highly polar compound, one of the most widely used quick-acting contact herbicides in the world, and is used for the control of agricultural weeds in many countries. Paraquat is both a herbicide and a highly toxic poison. Oral administration of paraquat to rats LD 50 It is 150mg / kg. Although it is listed as a moderately toxic poison in the toxicological classification, because there is no effective rescue drug at present, the oral mortality rate is 90%, and it should be listed as a hig...

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Problems solved by technology

[0005] Affected by its own physical and chemical properties, paraquat is difficult to retain on the reversed-phase chromatographic column, and there is great difficulty in concentration determination. The analysis and detection methods of paraquat include ultraviolet spectrophotometry, chromatography, capillary electrophoresis and other methods. Extraction methods also include liquid-liquid extraction, solid-phase extraction, etc., but the operation is cumbersome, time-consuming and laborious, which is not conducive to clinical practice

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