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Method for cultivating morchella strains

A method for cultivating Morchella, applied in directions such as botanical equipment and methods, cultivation, plant cultivation, etc., can solve problems such as poor stability of Morchella strains, avoid inconsistency between expression form and female parent, good growth, The effect of maintaining stability

Active Publication Date: 2019-10-08
南京金色种业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved in the present invention is to overcome the problem of poor stability of hickory chick bacterial classification in the prior art, thereby provide a kind of performance stable hickory chick bacterial classification cultivation method

Method used

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Embodiment 1

[0030] The present embodiment provides a kind of cultivation method of hickory chick bacterial classification, and concrete steps are as follows:

[0031] (1) Production and cultivation of mother species

[0032] A. Production of mother seed medium: Weigh 160g of peeled potatoes, 16g of glucose, 14g of agar, 0.5g of potassium dihydrogen phosphate, 0.4g of magnesium sulfate, and 5g of corn flour, peel the potatoes, wash them, cut them into pieces, add water to boil After 18 minutes, mash and filter to obtain the filtrate; add the above-mentioned quality of glucose, agar, potassium dihydrogen phosphate, magnesium sulfate and corn flour to the above-mentioned filtrate, heat until the agar is completely dissolved, centrifuge, take the supernatant, and put it at 0.1MPa Autoclave under pressure for 30 minutes, take out and cool to obtain the mother seed medium;

[0033] B. Cultivation of the parent species: remove the stipe from the fruiting body of the hickory chick that grows rob...

Embodiment 2

[0041] The present embodiment provides a kind of cultivation method of hickory chick bacterial classification, and concrete steps are as follows:

[0042] (1) Production and cultivation of mother species

[0043] A. Production of mother seed medium: Weigh 120g of peeled potatoes, 11g of glucose, 12g of agar, 0.4g of potassium dihydrogen phosphate, 0.3g of magnesium sulfate, and 3g of corn flour, peel the potatoes, wash them, cut them into pieces, add water and boil them After 15 minutes, mash it and filter to obtain the filtrate; add the above-mentioned quality of glucose, agar, potassium dihydrogen phosphate, magnesium sulfate and corn flour to the above-mentioned filtrate, heat until the agar is completely dissolved, centrifuge, take the supernatant, and put it at 0.1MPa Autoclave under pressure for 30 minutes, take out and cool to obtain the mother seed medium;

[0044] B. Cultivation of the parent species: remove the stipe from the fruiting body of the hickory chick that ...

Embodiment 3

[0052] The present embodiment provides a kind of cultivation method of hickory chick bacterial classification, and concrete steps are as follows:

[0053] (1) Production and cultivation of mother species

[0054] A. Production of mother seed medium: Weigh 180g of peeled potatoes, 18g of glucose, 16g of agar, 0.6g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, and 7g of corn flour, peel the potatoes, wash, cut into pieces, add water and boil After 20 minutes, mash and filter to obtain the filtrate; add glucose, agar, potassium dihydrogen phosphate, magnesium sulfate and corn flour to the above filtrate, heat until the agar is completely dissolved, centrifuge, take the supernatant, and put it under high pressure at 0.1MPa Sterilize for 30 minutes, take out and cool, and obtain the mother seed medium;

[0055] B. the cultivation of female species: the hickory chick sporocarps that grow robustly in the wild environment, are big, and grow well are removed the stipe,...

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Abstract

The invention belongs to the technical field of edible mushrooms and particularly relates to a method for cultivating morchella strains. The method includes production and cultivation of female species, original species and cultured species. With the method, each culture medium is adjusted in the cultivation process of the morchella strains, the preparation method of each culture medium is adjusted, and potassium dihydrogen phosphate, magnesium sulfate and corn flour are added to the culture medium, so that the female species can absorb more nutrient substances from the culture medium, and thesituation that the morphology of the strains is inconsistent with the female parent in the later breeding process to affect the yield is avoided.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a method for cultivating hickory chick strains. Background technique [0002] Morchella (Morchella), also known as Morchella, Morchella, etc., mycological classification belongs to the order of Panbacteriaceae, Morchellaceae, Morchella genus. It consists of a morel-shaped fertile capitula and a sterile stipe. The surface of the cap has a reticulated hymenium, and the edge is connected with the stipe. The stipe is cylindrical, hollow, and smooth. It may have grooves, and its shape resembles morel belly, so it gets its name. Morels are rich in 8 essential amino acids and vitamins, especially morels contain rare amino acids such as C-3-amino-L-proline, aminoisobutyric acid and 2,4-diaminoisobutyric acid , thus having its unique flavor, is one of the most famous and precious edible fungi in the world. Its artificial domestication and planting have bee...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/40
CPCA01G18/20A01G18/40
Inventor 杨平张边江王立科唐宁陈全战
Owner 南京金色种业科技有限公司
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