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Bio-drawing method for microstructure of Ganodorma lucidum

A technology of microstructure and structural diagrams, applied in the biological field, can solve problems such as low accuracy, inability to display structural features in the drawing tube, large errors, etc., to achieve the effect of ensuring accuracy

Active Publication Date: 2019-10-08
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, two methods have been used to map the microstructure of Ganoderma lucidum slices before: 1) Observing the microstructural features of Ganoderma lucidum in a microscope, and then drawing on white paper with short-term memory; the disadvantage of this method is that the accuracy is very low and the error is too large ; 2) While observing the microstructure characteristics of Ganoderma lucidum through a microscope, draw on white paper through the drawing tube carried by the microscope itself. The disadvantage of this method is that the definition of the drawing tube is very poor. Very strong, the drawing tube cannot display its structural features, and the drawing tube is an additional tool for the microscope, which is expensive; if the indoor light is poor, it is almost impossible to see the microstructural features of Ganoderma lucidum through the drawing tube

Method used

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Embodiment Construction

[0031] A method for biological mapping of ganoderma lucidum microstructure, specifically comprising the following steps:

[0032] (1) Prepare sliced ​​samples of Ganoderma lucidum: Hold the fruiting body of Ganoderma lucidum with the thumb, index finger, and middle finger of the left hand, select a section of the fruiting body of Ganoderma lucidum, hold the blade in the right hand steadily and keep it parallel to the section, and gently cut the surface of the section with the blade Flatten, then cut several slices continuously, select a flat and thin material and place it in the center of the glass slide, drop an appropriate amount of floating agent, and cover with a cover glass, press the cover glass lightly to squeeze out the air bubbles, so that the cover glass and Slides fit tightly.

[0033] (2) Microscopic observation, measurement and photographing: place the glass slide under a microscope and use a 5x mirror, a 10x mirror, a 40x mirror and an oil mirror (100x mirror) to...

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Abstract

The invention discloses a simple, practical and accurate bio-drawing method for the microstructure of Ganodorma lucidum, thereby meeting needs of scientific research. With the disclosed method, the drawing is made accurately; the operation is simple; no auxiliary device is needed; and the cost is low. According to the invention, the microstructure of the hymenium of the Ganodorma lucidum is measured under observation by the microscope; and under the microscope, the microstructure of Ganodorma lucidum is measured by using a measuring tool, a measuring scale is attached, and then shooting is performed for storage, so that the high accuracy of the microstructure ratio is ensured. The microstructure, observed by the microscope, of Ganodorma lucidum is drawn based on the original shape and scale by using a plotting instrument, so that the accuracy of the structure is enhanced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for biological mapping of ganoderma lucidum microstructure. Background technique [0002] Ganoderma lucidum (scientific name: Ganoderma lingzhi Sheng H.Wu, Y.Cao&Y.C.Dai): Also known as Lin Zhongling and Qiongzhen, it is a fungal fruiting body of the genus Ganoderma lucidum belonging to the fungal kingdom Basidiomycetes, Agaricomycetes, Polyporacea, Ganodermaceae. It has the effects of invigorating qi and calming the nerves, relieving cough and asthma, and prolonging life. For dizziness and insomnia, palpitation, shortness of breath, neurasthenia, fatigue and cough. [0003] Fruiting bodies of Ganoderma lucidum are annual, with lateral or partial stalks, cork when fresh, and cork when dry. The cap is 16×12 cm, about 2.6 cm thick, and the basidiocarp is light yellow to yellowish brown. The orifices are nearly round to polygonal, 5-6 per mm. The fungus flesh is double-laye...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84B41M5/00
CPCB41M5/00G01N21/84G01N2021/8466
Inventor 赵长林吴亚星杨占恩
Owner SOUTHWEST FORESTRY UNIVERSITY