Microbial agent for degrading fomesafen and preparation method and application thereof

A microbial inoculum and microcapsule technology, applied in the field of microorganisms, can solve the problem that there is no microbial inoculum that can effectively degrade fomesafen, and achieve the effect of significant degradation effect.

Active Publication Date: 2019-10-22
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that there is no effective microbial agent fo

Method used

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  • Microbial agent for degrading fomesafen and preparation method and application thereof
  • Microbial agent for degrading fomesafen and preparation method and application thereof
  • Microbial agent for degrading fomesafen and preparation method and application thereof

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Experimental program
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specific Embodiment approach 1

[0052] Specific embodiment one: a kind of microcapsule bacterial preparation of the present embodiment, the bacterium used in described microcapsule bacterial preparation is Bacillus Velezensis (Bacillus Velezensis) FB11 and oligooxymonas (Stenotrophomonas sp.) The mixed bacteria of FB14; among them,

[0053] Bacillus Velezensis (Bacillus Velezensis) FB11, preserved in the General Microbiology Center of the China Committee for the Collection of Microorganisms, the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation date is May 08, 2019. The deposit number is CGMCC No.17732;

[0054] Stenotrophomonas sp. FB14, preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms, the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation date is May 08, 2019 , the deposit number is CGMCC No.17731.

specific Embodiment approach 2

[0055] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the microcapsule bacterial agent is a mixture of Bacillus Velezensis FB11 and Stenotrophomonas sp. FB14 bacteria, sodium alginate, activated carbon and calcium chloride; wherein, the mass ratio of Bacillus Velezensis FB11 to Stenotrophomonas sp. FB14 is 1:1.

[0056] Others are the same as in the first embodiment.

specific Embodiment approach 3

[0057] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the mass percentage of mixed bacteria in the microcapsule microbial agent is 92%, the mass percentage of sodium alginate is 2%, and the mass percentage of activated carbon The content is 5%, and the mass percent content of calcium chloride is 1%.

[0058] Others are the same as in the first embodiment.

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Abstract

The invention discloses a microbial agent for degrading fomesafen and a preparation method and application thereof, and belongs to the field of microorganisms. The microbial agent aims at solving theproblem that currently, there is no microbial agent for effectively degrading fomesafen; bacteria adopted in the microcapsule microbial agent are mixed bacteria of bacillus velezensis FB11 and stenotrophomonas sp. FB14, the preservation numbers of the bacteria are CGMCC No.17732 and CGMCC No.17731 respectively, the preservation dates are both May 8th, 2019, and the preservation address is BuildingNo.3, NO.1 West Beichen Road, Chaoyang District, Beijing. After the two strains are mixed, auxiliary materials are added to prepare the microbial agent. The microbial agent is used for degrading theherbicide fomesafen. The mixed strains adopted for the microbial agent have a remarkable degradation effect on the herbicide fomesafen in soil.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for preparing a microbial agent for degrading fomesafen. Background technique [0002] The chemical name of fomesafen is 5-(2-chloro-4-trifluoromethylphenoxy)-N-methylsulfonyl-2-nitrobenzamide, which belongs to the herbicides of diphenyl ethers. It has high herbicidal activity at low concentration, so it has received more and more attention in agricultural weed control. [0003] When using fomesafen aqueous solution at a concentration of 250g / L in the field, the dosage is very small, usually 667m 2 / 100mL, but at this dosage, fomesafen has a good control effect on annual broad-leaved weeds, and it is mainly used in the fields of soybeans, corn, cotton, peanuts and other crops. However, due to its long residual time in the soil, generally up to 6-12 months, that is to say, when planting crops in the second year, there are still a large amount of herbicides from the previou...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/10C12N11/14B09C1/10C12R1/07C12R1/01
CPCB09C1/10C12N1/20C12N11/10C12N11/14
Inventor 刘春光杨峰山耿贵付海燕曾伟民鲁红刚马玉堃孙丛何婧王家瑞
Owner HEILONGJIANG UNIV
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