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Microbacterium PT3 with protein degradation function and application thereof

A microbacterium, PT3 technology, applied in the field of microorganisms, can solve the problems of low digestibility of plant protein, inability to decompose and transform, low utilization rate of feed protein, etc.

Active Publication Date: 2020-06-26
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Researchers have improved the palatability of aquatic feed, added appropriate attractants, and adjusted the balance of amino acids in feed to increase the feed intake of farmed animals. However, in order to keep costs down, most of the protein in domestic feed is plant protein. , Aquaculture animals have low digestibility of these plant proteins, which cannot be completely decomposed and transformed by the digestive enzymes of aquatic animals, which is also the main limiting factor for the low utilization rate of feed protein

Method used

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  • Microbacterium PT3 with protein degradation function and application thereof
  • Microbacterium PT3 with protein degradation function and application thereof
  • Microbacterium PT3 with protein degradation function and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1 Enrichment and screening of microbacterium PT3 that can secrete protease extracellularly

[0024] Sample source: Litopenaeus vannamei aquaculture water body in intelligent mariculture room of South China Sea Institute of Oceanology, Chinese Academy of Sciences.

[0025] The specific implementation steps are as follows: select healthy prawns with large vitality and full digestive tract in the prawn breeding pond, and bring them back to the laboratory immediately. The remaining seawater on the surface of the prawns was absorbed by filter paper, the body surface was wiped with 75% v / v alcohol for disinfection, dissected under aseptic conditions, and the complete intestinal tract was taken out. Rinse the intestinal tissue with sterile PBS (pH=7.4) buffer solution for 3 to 4 times, then put it into a 1.5mL sterile EP tube, add 1mL of PBS buffer solution to the sterile EP tube, and grind it evenly with a sterile grinding rod. Bacterial suspension was prepared by 10...

Embodiment 2

[0026] The identification of embodiment 2 microbacteria PT3 bacterial classification

[0027] 1. Morphological observation

[0028] The purely cultured PT3 strain was inoculated on the surface of 2216E solid medium by streaking method, and cultured in a 30°C incubator for 24 hours to observe the morphological characteristics of the colony. The colony is dot-shaped, opaque white, with smooth edges, and the colony is moist.

[0029] The 2216E solid medium is prepared by adding agar powder with a mass fraction of 1.5% to the 2216E liquid medium components, mixing and sterilizing. The 2216E liquid medium contains 5g of peptone per liter, 1g of yeast extract powder, 0.1g of ferric citrate, 19.45g of sodium chloride, 5.98g of magnesium chloride, 3.24g of sodium sulfate, 1.8g of calcium chloride, and 0.55g of potassium chloride , sodium carbonate 0.16g, potassium bromide 0.08g, strontium chloride 0.034g, boric acid 0.022g, sodium silicate 0.004g, sodium fluoride 0.0024g, ammonium n...

Embodiment 3

[0041] Example 3 Exploration of Optimal Growth Conditions for Microbacterium PT3

[0042] The effects of different pH, temperature and NaCl mass fraction (salinity) on the growth of Microbacterium PT3 were studied respectively. Among them, the different temperatures are: 20°C, 25°C, 30°C, 37°C, 43°C; the different pH values ​​are: 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0; the different NaCl mass fractions are: 0 %, 1%, 2%, 3%, 4%, 5%.

[0043] In addition to the factors investigated in the above experiments, the fermentation conditions are to inoculate the microbacterium PT3 culture solution in the LB fermentation medium with an inoculation amount of 1% (v / v), and after cultivating at 30°C and 200rpm for 24 hours, take samples to measure the conditions OD 600 Value, the LB fermentation medium without inoculation was used as blank control. Take culture temperature, pH value, NaCl mass fraction as abscissa, OD 600 Values ​​plot the respective curves on the ordinate. see results ...

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Abstract

The invention discloses a microbacterium PT3 with a protein degradation function and application thereof. The preservation number of the microbacterium PT3 is GDMCC No: 60923. The microbacterium PT3 is separated from the intestinal tract of a prawn, and the proving result shows that the microbacterium PT3 can secrete protease extracellular, hydrolyze protein in a living environment, assist the microbacterium PT3 in utilizing the protein after being planted in the intestinal tract of an aquaculture animal, decompose the remaining protein in residual bait, synthesize the mycoprotein of the microbacterium PT3 for the aquaculture animal to use, and plant the microbacterium PT3 in the intestinal tract again to form a benign cycle and improve the feed protein utilization path. The microbacteriumand artemia survival experiments show that the strain does not generate stress or toxic effects on the aquaculture animal when the concentration is high; the strain is wide in tolerance, the salinityhas small influence on the growth of the bacterium, and the strain is very suitable for aquaculture and can efficiently play a role; and the microbacterium PT3 is of great significance in the marineaquaculture industry, can serve as a good strain of a novel microecological preparation and has good application prospects.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a microbacterium PT3 with protein degradation function and its application. Background technique [0002] With the development of the economy and the improvement of people's material living standards, the demand for aquatic products is increasing day by day. At present, my country is one of the important aquatic product production and export countries in the world. From January to November 2018 alone, the national aquatic product output has reached 53.7555 million tons. In order to increase the weight gain of farmed animals between units, the protein content in aquatic feed is always maintained at a high level, while the utilization rate of protein in feed for farmed animals is generally low, and the bait fed is only 25% feed protein It is absorbed and utilized by farmed animals, and the remaining 75% exists in the remaining bait or is discharged into the cultured water body w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P21/00A23K10/18A23K50/80C12R1/01
CPCA23K10/18A23K50/80C12N1/20C12P21/00C12N1/205C12R2001/01
Inventor 陈偿丁雄祺谢媚杨艺滢方建相管云雁云龙
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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