Hybridoma cell strain JS secreting anti-phenolphthalein monoclonal antibody and application of hybridoma cell strain JS

Abstract

The invention discloses a hybridoma cell strain JS secreting an anti-phenolphthalein monoclonal antibody and application of the hybridoma cell strain JS, and belongs to the field of food safety immunodetection. The hybridoma cell strain JS secreting the anti-phenolphthalein monoclonal antibody is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is March 7, 2019, and the preservation number is CGMCC No.17394. According to the hybridoma cell strain JS secreting the anti-phenolphthalein monoclonal antibody and the application of the hybridoma cell strain JS, a complete antigen of phenolphthalein and a Freund's adjuvant with the amount the same as that of the complete antigen are subjected to mixed emulsification, and a BALB / c mouse issubjected to subcutaneous multi-point injection at the nape part for being immunized. An indirect competitive euzymelinked immunosorbent assay is adopted for screening cells, subcloning is conductedthree times, and finally, the monoclonal antibody hybridoma cell strain is obtained. The monoclonal antibody secreted by the cell strain has good specificity and detection sensitivity (the IC50 valueis 15.4 ng / mL) for the phenolphthalein, the amount of phenolphthalein residuals in healthcare food can be detected, the raw material is provided for immunodetection for the phenolphthalein residuals in food, and the hybridoma cell strain JS secreting the anti-phenolphthalein monoclonal antibody has practical application value.

Description

technical field [0001] The invention relates to a hybridoma cell line JS that secretes anti-phenolphthalein monoclonal antibody and its application, which belongs to the field of food safety immune detection. Background technique [0002] Phenolphthalein (Phenolphthalein) is a weak organic acid, which is white or slightly yellowish fine crystals at room temperature. It has multiple uses. Phenolphthalein is colorless in acidic solution and red in lye or alkali metal carbonate solution. It is often used as an indicator of acid-base titration in chemical experiments. In organic synthesis, it is mainly used to synthesize plastics, especially phthalazinone polyaryletherketone polymers. [0003] Phenolphthalein is used as a contact laxative in medicine, which stimulates the colonic mucosa to strengthen and organize the absorption of intestinal fluid, and accumulates water and electrolytes in the colon to produce a laxative effect and promote defecation. It is clinically used as ...

AI Technical Summary

Problems solved by technology

These detection methods all have the disadvantages of high detection cost, long time-consuming and high professional quality requirements for detection personnel.

In addition, it is also reported that phenolphthalein is colorless in acidic and neutral solutions and pink in alkaline environments with pH > 10 to prepare a phenolphthalein detection card for detection. Although this method is simple to operate, it is convenient Carry but has the disadvantage of poor accuracy and cannot be quantitatively analyzed

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