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Application and kit of reagents for detecting expression level of open reading frame 53 of chromosome 8

An open reading frame and expression level technology, applied in the biological field, can solve the problems that limit the wide application of breast cancer prognostic markers

Active Publication Date: 2021-11-30
JIERUI BIOENG SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Breast cancer exhibits great complexity and heterogeneity in terms of cell origin, histological morphology, disease grade, clinical manifestations, treatment response, and metastatic potential, which limits the wide application of breast cancer prognostic markers

Method used

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  • Application and kit of reagents for detecting expression level of open reading frame 53 of chromosome 8
  • Application and kit of reagents for detecting expression level of open reading frame 53 of chromosome 8
  • Application and kit of reagents for detecting expression level of open reading frame 53 of chromosome 8

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This example is used to illustrate the TCGA high-throughput data analysis of the expression changes of C8ORF53 in breast cancer.

[0029] 1. TCGA high-throughput data analysis process:

[0030] Log in to the TCGA portal UALCAN ( http: / / ualcan.path.uab.edu / index.html ) home page, click "Analysis", enter the gene name "C8ORF53", select TCGA dataset "Breast invasive carcinoma", search, click "Expression", and record the result. GraphPad 12.0 software was used to draw graphs, and the statistical method was T test, and P<0.05 was considered statistically significant.

[0031] 2. Results: The expression of C8ORF53 in breast cancer tissue was significantly higher than that in normal tissue (Pfigure 1 shown.

Embodiment 2

[0033] This example is used to illustrate the relationship between the high expression of C8ORF53 and the prognosis of breast cancer through TCGA high-throughput data analysis.

[0034] 1. TCGA high-throughput data analysis process:

[0035] Log in to the TCGA portal cBioPortal (http: / / www.cbioportal.org / ), select the tumor dataset "Breast Invasive Carcinoma (TCGA Provisional)", select "mRNA Expression z-Scores (RNA Seq V2RSEM)" for the omics data, and select the gene Enter "C8ORF53: EXP>=2", search, click "Survival" on the pop-up page, and record the result. Survival curves were drawn by Kaplan-Meier method, and the differences in survival curves were tested by log-rank test. P<0.05 was considered statistically significant.

[0036] 2. Results: High expression of C8ORF53 is not conducive to the overall survival of breast cancer patients (P=0.0127) ( figure 2 ).

Embodiment 3

[0038] This example is used to illustrate the preparation of reagents for detecting the expression of C8ORF53 and the preparation of kits for the prognosis of breast cancer patients (50 reactions).

[0039] 1. Trizol 50.0ml;

[0040] 2. Isopropanol 50.0ml;

[0041] 3. Chloroform 50.0ml;

[0042] 4. 50.0ml absolute ethanol;

[0043] 5. RNase-free water 5.0ml

[0044] 6. 1.0μM random primers (Random primers) 50.0μl;

[0045] 7. 2.0ml of 5×M-MLV buffer;

[0046] 8. 100.0μl of 10.0mM deoxynucleotide triphosphates (dNTPs);

[0047] 9. 40U / μl RNase inhibitor 50.0μl;

[0048] 10. 200U / μl M-MLV reverse transcriptase 50.0μl;

[0049] 11. ABI 2×PCR Mix 2.0ml;

[0050] 12. 10.0 μM C8ORF53 real-time fluorescent quantitative PCR specific primer 30.0 μl, the primer sequence is shown in Table 1;

[0051] 13. 30.0 μl of specific primers for 10.0 μM ACTB real-time fluorescence quantitative PCR. See Table 1 for the primer sequences.

[0052] Table 1 Fluorescence quantitative RT-PCR prim...

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Abstract

The invention belongs to the field of biotechnology, and relates to the application and kit of a reagent for detecting the expression level of the open reading frame 53 of chromosome 8. More specifically, it relates to the application of a reagent for detecting the expression level of C8ORF53 in the preparation of a preparation for auxiliary diagnosis of breast cancer and / or judgment of prognosis of breast cancer patients and a preparation for auxiliary diagnosis of breast cancer and / or judgment of prognosis of breast cancer patients Reagent test kit. The detection results of clinical samples showed that the expression of C8ORF53 in breast cancer was significantly higher than that in adjacent tissues; and the high expression of C8ORF53 was not conducive to the overall survival of breast cancer patients. Therefore, the reagent for detecting the change of the gene expression can be used for the prognosis, diagnosis and treatment of breast cancer.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to the application of a reagent for detecting the expression level of chromosome 8 open reading frame 53 (C8ORF53) in the preparation of preparations for breast cancer auxiliary diagnosis and / or prognosis judgment of breast cancer patients and a A kit for auxiliary diagnosis of breast cancer and / or prognosis judgment of breast cancer patients. Background technique [0002] Breast cancer is the most common gynecological malignancy. Global cancer epidemiological statistics show that in 2012, the total number of new cases of breast cancer worldwide was 1,677,000. The incidence of breast cancer in my country is as high as 25.89 / 100,000, accounting for about 16.83% of all female malignant tumors. Moreover, the number of breast cancer patients in my country and the proportion of newly diagnosed patients in the world are increasing year by year, and there is a trend of younge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/68G01N33/574
CPCC12Q1/6886C12Q2600/118C12Q2600/158G01N33/57415G01N33/57484G01N33/68G01N2333/47G01N2800/52
Inventor 樊伟平夏伟吴隽松胡明李春明张虎
Owner JIERUI BIOENG SHANGHAI