Apparatus and methods for continuous diagnostics of macromolecules

Abstract

Devices, apparatus and methods for nanopore sequencing macromolecules are disclosed. The devices and apparatus include independently controllable fluid channels (101, 104) bi-directionally coupled toa measurement module (100). By flowing a lipid-containing solution through the channels (101, 104), a lipidic bilayer nanopore (111) can be assembled in the measurement module (100).

Description

[0001] This application claims the benefit of U.S. Provisional Application No. 62 / 458,840, filed February 14, 2017, which is hereby incorporated by reference in its entirety, under Title 35, United States Code, Section 119(e). technical field [0002] The present invention relates to devices, devices and methods for sequencing macromolecules. The apparatus and apparatus include independently controllable fluid channels bi-directionally coupled to a measurement module. Background technique [0003] Solid-state nanopores and biological nanopores are increasingly becoming the focus of developing low-cost high-throughput macromolecular sequencing systems. The approach to nanopore-based sensing employs the measurement of ion current flow through nanopores incorporated within a high resistance amphiphilic membrane between electrodes provided on either side of the membrane. The flow of ion currents through the nanopore is regulated by the different nucleotide bases of the DNA stra...

AI Technical Summary

Problems solved by technology

Variations in the mass of the device in a module can lead to poor signal-to-noise ratios, while differences in osmolality of solutions on either side of a membrane containing nanopores can cause reliability issues

Furthermore, the methods used to control the rate of movement of macromolecules across nanopores can be less than ideal, leading to high error rates

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