A kind of anti-tumor platinum drug mineralized protein nanoparticle and its preparation method and application

A technology of platinum-based drugs and nanoparticles, which is applied in the field of biomedicine to achieve the effects of uniform size, high tumor targeting and controllable particle size

Active Publication Date: 2021-11-09
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in this field, there is an urgent need to develop a new type of platinum-based drug protein nanoparticle with clinical application prospects and tumor targeting, so as to realize the tumor-targeted delivery of platinum-based drugs and overcome the resistance of platinum-based drugs. to improve the anti-tumor effect of platinum-based drugs

Method used

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  • A kind of anti-tumor platinum drug mineralized protein nanoparticle and its preparation method and application
  • A kind of anti-tumor platinum drug mineralized protein nanoparticle and its preparation method and application
  • A kind of anti-tumor platinum drug mineralized protein nanoparticle and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Preparation of platinum-based drug mineralized protein nanoparticles, the specific steps are as follows:

[0056] Synthesis of platinum-based prodrug compounds: After mixing cisplatin and silver nitrate in water at a molar ratio of 1:2, react in the dark at 60°C for 3 hours, then cool down to room temperature, continue to react for 10 hours, and then react at 12000 rpm The sample was centrifuged at a rotating speed for 12 minutes to remove the silver chloride precipitate, and the obtained supernatant was an aqueous solution of platinum-based prodrug compound.

[0057] Take 100 mM platinum-based prodrug compound aqueous solution and 13.3 mg / mL human serum albumin (HSA) aqueous solution and mix thoroughly according to the volume ratio of 1:5, the molar ratio of platinum-based prodrug compound to human serum albumin is 100:1 , use 0.2 M NaOH solution to adjust the pH of the mixed solution to 6.4, then add potassium iodide (KI) aqueous solution to introduce ligand ion iodid...

Embodiment 2

[0061] The steps of this example are the same as in Example 1, the difference is that the molar ratio of the platinum prodrug compound to the ligand ion is 1:4, and the prepared nanoparticles are photographed with an electron microscope, and the results are as follows figure 2 As shown, the average particle size of the prepared nanoparticles was 4.1±0.3 nm.

Embodiment 3

[0063] The steps of this example are the same as in Example 1, the difference is that the molar ratio of the platinum prodrug compound to the ligand ion is 1:2, and the prepared nanoparticles are photographed with an electron microscope, and the results are as follows image 3 As shown, the average particle size of the prepared nanoparticles was 2.3±0.2 nm.

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Abstract

The invention discloses an anti-tumor platinum drug mineralized protein nanoparticle, a preparation method and application thereof. The present invention adopts a two-step preparation method for the first time to successfully prepare platinum-based drug mineralized protein nanoparticles, and the prepared nanoparticles are uniformly dispersed in an aqueous solution; the nanoparticles have good tumor cytotoxicity and are effective against human non-small cell lung cancer cells A549 IC 50 It was 6.9 μg / ml. The platinum-based drug mineralized protein nanoparticles provided by the present invention include platinum-based drugs and proteins, and its remarkable advantages are simple preparation process, uniform size, controllable particle size, good biocompatibility, good water solubility, and long circulation time in blood. Long, high tumor targeting characteristics, laid the foundation for efficient tumor treatment and drug-resistant tumor treatment.

Description

technical field [0001] The invention belongs to biomedicine technology, and in particular relates to a platinum drug mineralization protein nanoparticle and its preparation method and application. Background technique [0002] Platinum drugs are commonly used clinical anti-tumor chemotherapy drugs, which have broad-spectrum anti-tumor effects. Platinum drugs also have many deficiencies, such as poor curative effect, high toxicity and side effects, poor targeting, and prone to tumor drug resistance. Nano-drug carriers (such as polymer micelles, vesicles, etc.) can improve this. However, platinum-based antineoplastic drugs commonly used in clinical practice (such as cisplatin and its derivatives) are difficult to pass through due to their poor water and oil solubility. Traditional nanocarrier entrapment usually uses chemical coupling or prodrug modification to achieve its loading in the carrier, but these nano-drug carriers often have disadvantages such as difficult to effect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K9/52A61K31/555A61K33/243A61K47/42A61P35/00
CPCA61K9/0019A61K9/5169A61K31/555A61P35/00A61K33/243
Inventor 陈华兵邓益斌杨红柯亨特刘玲李厅杨涛王璐
Owner SUZHOU UNIV
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