Method for in-situ characterization of bioavailability of hydrophobic organic pollutants in soil microdomains

A technology of organic pollutants and bioavailability, applied in the field of environmental science and engineering, can solve the problems of bioavailability of organic substances that cannot be hydrophobic, and achieve stable and reliable results with low theoretical accuracy

Pending Publication Date: 2019-11-05
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Aiming at the problem that the prior art cannot characterize the bioavailability of hydrophobic organic matter in soil micro-domains, the present invention adopts the method of in-situ culture of micron-scale, strongly hydrophobic polydimethylsiloxane probes to analyze the bioavailability of hydrophobic organic matter in soil micro-scale The distribution and enrichment of hydrophobic organic pollutants can realize the in-situ accurate characterization of hydrophobic organic pollutants in soil micro-domains

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  • Method for in-situ characterization of bioavailability of hydrophobic organic pollutants in soil microdomains
  • Method for in-situ characterization of bioavailability of hydrophobic organic pollutants in soil microdomains
  • Method for in-situ characterization of bioavailability of hydrophobic organic pollutants in soil microdomains

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Embodiment 1

[0043] 1) Using the method of the present invention to adopt polydimethylsiloxane probes to in-situ cultivate and enrich polycyclic aromatic hydrocarbons in the soil, and the specific process of detecting the enriched polycyclic aromatic hydrocarbons is as follows:

[0044] a) Weigh 5g of polycyclic aromatic hydrocarbon-contaminated red soil in a 150mL transparent beaker, vibrate and lay flat;

[0045] b) Take three polydimethylsiloxane probes with a length of 4 cm and place them on the upper surface of the red soil at the bottom of the beaker. The polydimethylsiloxane probes are commercially available from Poly Micro Technologies, Inc., USA The probe, the mass ratio of the polydimethylsiloxane probe to the soil is less than one thousandth, the diameter of the polydimethylsiloxane probe is not greater than 110 μm, and the probe is in the soil The depth is greater than 1mm.

[0046] c) Weigh again 5g of PAH-contaminated red soil and place it above the polydimethylsiloxane prob...

Embodiment 2

[0059] The basic content of this embodiment is the same as embodiment 1, the difference is:

[0060] 1) Using the method of the present invention to use polydimethylsiloxane probes to in-situ cultivate and enrich polycyclic aromatic hydrocarbons in the soil, and the specific process of detecting the enriched polycyclic aromatic hydrocarbons is basically the same as that of Example 1, except that in:

[0061] a) Weigh 30g of polycyclic aromatic hydrocarbon-contaminated red soil in a 150mL transparent beaker, vibrate and lay flat;

[0062] b) Take 10 polydimethylsiloxane probes with a length of 4 cm and place them on the upper surface of the red soil at the bottom of the beaker. The polydimethylsiloxane probes are commercially available from Poly Micro Technologies in the United States. the probe;

[0063] c) Weigh again 30g of polycyclic aromatic hydrocarbon-contaminated red soil and place it above the polydimethylsiloxane probe, oscillate and shake well, completely cover the...

Embodiment 3

[0079] This example is the specific operation of the bioavailability characterization experiment of polycyclic aromatic hydrocarbons in the rhizosphere area:

[0080] 1) Weigh 2.5kg of PAHs-contaminated soil (dry weight), divide it into three treatment groups, namely blank (CK), add 1% corn straw charcoal, add 1% wheat straw charcoal, mix well, and load them layer by layer image 3 In the shown root box, the width of each layer along the horizontal direction is 1mm, and each layer of soil is covered with seven 4cm polydimethylsiloxane probes according to the method in Example 1, and the arrow position is the root growth chamber ,Such as image 3 Probes were embedded at the rhizosphere, proximal rhizosphere and distal rhizosphere regions shown; water was added to 60% of the maximum water holding capacity of the soil;

[0081] 2) Take germinated ryegrass seeds, place them on the surface soil of the root growth room, spread a layer of dry soil, and treat them in the dark for 2 d...

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Abstract

The invention discloses a method for in-situ characterization of the bioavailability of hydrophobic organic pollutants in soil microdomains, and belongs to the technical field of environmental scienceand engineering. The method comprises the steps of placing a micro-scale polydimethylsiloxane probe into a soil medium; enriching the hydrophobic organic pollutants in soil in an in-situ culture mode, and extracting and measuring the hydrophobic organic pollutants in the probe. The method utilizes the micro-scale and strong hydrophobicity characteristics of the polydimethylsiloxane probe, can simulate the enrichment of organisms in the hydrophobic organic pollutants on the basis of in-situ non-interference distribution of the hydrophobic organic pollutants in the soil, realizes the in-situ characterization of the bioavailability of the hydrophobic organic pollutants in the soil microdomains, and provides a technical support for further exploring the migration, transformation and degradation mechanisms of the hydrophobic organic pollutants at the interfaces of animal activity / plant root system-soil microdomains.

Description

technical field [0001] The invention relates to the field of environmental science and engineering technology, more specifically, to a method for in-situ characterization of the bioavailability of hydrophobic organic pollutants in soil micro-domains. Background technique [0002] With the rapid development of society, the interference of human activities such as the synthesis and use of a large number of chemicals, the discharge of industrial wastes, the incomplete combustion of coal and oil, etc., the environment has been polluted to varying degrees on a global scale, which seriously threatens and endangers the ecology. Safety. Among them, hydrophobic organic pollutants have attracted much attention due to their persistence, long-distance migration, bioaccumulation, and three effects (carcinogenic, teratogenic, and mutagenic), and have become the top priority of research and governance. Polycyclic aromatic hydrocarbons, polychlorinated biphenyls and phthalates have been li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/08
CPCG01N30/02G01N30/08
Inventor 宋洋程虎卞永荣蒋新
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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