Unlock instant, AI-driven research and patent intelligence for your innovation.

Natural nano-vesicle and preparation method and applications thereof

A nanovesicle, a natural technology, applied in animal cells, digestive system, medical raw materials derived from mammals, etc., can solve problems such as unclear roles and invasion

Pending Publication Date: 2019-11-08
THE FIRST PEOPLES HOSPITAL OF CHANGZHOU
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of exosomes derived from HCC cells in the invasion and metastasis of HCC in vitro and in vivo is still unclear. This study attempts to explore this issue and further explore whether there are differences in the role of exosomes derived from HCC cells with different invasion characteristics. and specific molecular mechanisms
[0006] At present, there is a lack of an effective, high-yield and well-defined natural nanovesicles and their preparation methods and applications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Natural nano-vesicle and preparation method and applications thereof
  • Natural nano-vesicle and preparation method and applications thereof
  • Natural nano-vesicle and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The application of the natural nanovesicle of the present invention in the preparation of inhibitors for regulating cell transfer and TGF-β signaling pathway.

[0029] The application of the natural nanovesicle of the present invention in the preparation of various acute and chronic liver diseases or liver damage drugs or inhibitors caused by acute and chronic liver diseases.

[0030] The application of a natural nanovesicle of the present invention in the preparation of drugs or inhibitors of HCC primary liver cancer.

[0031] The cells are exosomes derived from HCC primary liver cancer cells.

[0032] The exosomes derived from HCC primary liver cancer cells promote the invasion and metastasis of HCC in vivo.

[0033] The exosomes derived from HCC primary liver cancer cells can activate recipient cells by transporting fibroblast growth factor FGF, vascular endothelial growth factor VEGF and epidermal growth factor EGF to affect cell growth and migration.

[0034] The...

Embodiment 2

[0043] The difference between embodiment 2 and embodiment 1 is:

[0044] The preparation method of the natural nanovesicles of the present invention comprises the following steps: (1) extraction of exosomes: the extraction of exosomes in patient serum adopts ExoquickTM Kit (System Biosciences, USA) method; ExoquickTM Kit (System Biosciences , USA) The steps of the method are as follows: 50 μl of serum was centrifuged at 5000 g for 20 minutes to remove cells and debris, the supernatant was transferred to another clean and sterilized tube, 20 μl of Exoquick TM Kit reagent was added and mixed up and down, incubated at 4 °C, centrifuged at 1500 g for 20 Minutes, an off-white precipitate can be seen at the bottom of the tube, remove the supernatant and centrifuge again at 1500g for 20 minutes, carefully remove the supernatant, and resuspend the pellet with PBS buffer or RNA lysate according to the subsequent use. The extracted precipitate was observed by transmission electron micro...

Embodiment 3

[0049] The difference between embodiment 3 and embodiment 1 is:

[0050] The preparation method of the natural nanovesicles of the present invention comprises the following steps: (1) extraction of exosomes: the extraction of exosomes in patient serum adopts ExoquickTM Kit (System Biosciences, USA) method; ExoquickTM Kit (System Biosciences , USA) The steps of the method are as follows: 300 μl of serum was centrifuged at 4000g for 10 minutes to remove cells and debris, the supernatant was transferred to another clean sterile tube, 100 μl of Exoquick™ Kit reagent was added and mixed upside down, incubated at 4°C, centrifuged at 1500g for 10 Minutes, an off-white precipitate can be seen at the bottom of the tube, remove the supernatant and centrifuge again at 1500g for 30 minutes, carefully remove the supernatant, and resuspend the pellet with PBS buffer or RNA lysate according to the subsequent use. The extracted precipitate was observed by transmission electron microscope acco...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a natural nano-vesicle and a preparation method and applications thereof. The invention also discloses an application of the natural nano-vesicle in preparation of an inhibitorfor regulating and controlling cell transfer and a TGF-beta signal pathway. The invention relates to an application of the natural nano vesicle in preparation of a medicine or an inhibitor for treating various acute and chronic liver diseases or liver injuries caused by the acute and chronic liver diseases. The invention relates to an application of the natural nano vesicle in preparation of a medicine or an inhibitor for HCC primary liver cancer.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a natural nanovesicle and its preparation method and application. Background technique [0002] Exosomes are nanoscale biological vesicles released by a variety of cells (including tumor cells), containing a variety of active cytokines, proteins and genetic materials (mRNA, DNA and miRNA). In this study, we examined the role and molecular mechanism of exosomes derived from liver cancer cell lines and normal liver cells with different invasive characteristics in the EMT of epithelial phenotype hepatocellular carcinoma cells. The results of the study found that exosomes derived from HCC cells could Promote the migration and invasion of recipient cells, induce the reduction of E-cadherin expression in recipient cells, increase the expression of Vimentin and EMT, and the effect of exosomes derived from high-invasive HCC cells is stronger than that of low-invasive HCC cells...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/13A61P1/16A61P35/00C12N5/09
CPCA61K35/13A61P1/16A61P35/00C12N5/0693
Inventor 屈振
Owner THE FIRST PEOPLES HOSPITAL OF CHANGZHOU