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Fingerprint of preparation for muscle relaxing and blood circulation stimulation and application thereof in overall quality evaluation

A technology for relaxing tendons and promoting blood circulation and fingerprinting, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problem of ignoring the synergy of multiple mechanisms of the diversity of traditional Chinese medicine components, difficult to comprehensively measure the efficacy and quality of traditional Chinese medicine, and the difficulty of quantitative indicators to meet quality standards. and other problems to achieve the effect of quality control, high column efficiency and pressure reduction

Inactive Publication Date: 2019-11-08
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And there are certain problems in the current ministerial quality standards. At present, there are only thin-layer chromatography to qualitatively identify Cyperus cyperi, safflower, dog ridge and xiangjiapi, and HPLC to determine the content of 4-methoxy salicylaldehyde
However, the formula of this variety is complex and the ingredients are diverse, and a single quantitative index is difficult to meet the requirements of its quality standard.
[0004] The current quality standards only control some of the ingredients in traditional Chinese medicine, ignoring the diversity of ingredients in Chinese medicine and the synergy of multiple mechanisms, making it difficult to comprehensively measure the efficacy and quality of Chinese medicine

Method used

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  • Fingerprint of preparation for muscle relaxing and blood circulation stimulation and application thereof in overall quality evaluation
  • Fingerprint of preparation for muscle relaxing and blood circulation stimulation and application thereof in overall quality evaluation
  • Fingerprint of preparation for muscle relaxing and blood circulation stimulation and application thereof in overall quality evaluation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Optimization of liquid chromatography detection conditions for Shujinhuoxue preparation:

[0038] The present invention is suitable for methanol-water-acetic acid (volume ratio 47:50:3), acetonitrile-0.1vt% phosphoric acid aqueous solution, acetonitrile-0.2vt% phosphoric acid aqueous solution, acetonitrile-0.2vt% formic acid aqueous solution, methanol-0.2vt% formic acid aqueous solution, etc. Different ratios and types of elution systems were screened, and different wavelengths of 264nm, 270nm, 277nm, and 280nm were investigated. When the wavelength was 277nm, and the mobile phase was acetonitrile-0.2vt% formic acid aqueous solution, the separation of chromatographic peaks was higher, and the peak The total number is more, the peak purity is better, more components can be separated and the content is higher.

[0039] In the present invention, different extraction methods for relaxing muscles and tendons and activating blood circulation: heating reflux and ultrasound, sc...

Embodiment 2

[0041] Construction of fingerprints of a Shujinhuoxue preparation and its application in overall quality evaluation

[0042] The preparation for relaxing tendons and activating blood circulation is made from 10 medicinal materials, including safflower, Cyperus cyperi, dog ridge, mistletoe, shenjincao, eupatorium leaves, cypress bark, Caulis Spatholobus, Spathiphyllum vine, and natural copper. Its dosage forms include: Common pharmaceutical dosage forms such as tablets and capsules. According to the requirements of the fingerprint, the peak area of ​​ten common peaks was selected to be greater than 90% of the total peak area, and 6 chromatographic peaks were identified by mass spectrometry, wherein No. 4 peak was Cortex Phellodendron Lactone, No. 5 peak was hydroxysafflower yellow A, Peak No. 6 is phenylhexanoic acid, peak No. 8 is norsarcoside, peak No. 9 is rosmarinic acid, and peak No. 10 is 4-methoxy salicylaldehyde. Standards available on the market include hydroxysafflor...

Embodiment 3

[0080] The chemical pattern recognition analysis of Shujinhuoxue preparation comprises the following steps:

[0081] (1) Principal Component Analysis (PCA)

[0082] PCA analysis was carried out on 23 batches of Shujinhuoxue preparations, using SIMCA 13.0 statistical software to select the top three principal components with the largest contribution rate, taking the peak area of ​​10 common peaks as variables, and the total contribution rate of the first three principal components is 71.08% , that is, the first three principal components can reflect the basic characteristics of the Shujinhuoxue preparation. Calculate the principal component scores of 23 batches of Shujinhuoxue preparations, the principal component scores of 23 batches of samples are as follows: Figure 4 As shown, the loading diagrams of 10 indicators in 23 batches of samples are as follows Figure 5 shown.

[0083] Depend on Figure 4 It can be seen that it can be divided into three categories, S1, S2, S3,...

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Abstract

The invention, which belongs to the field of drug quality control, discloses a fingerprint of preparation for muscle relaxing and blood circulation stimulation and application thereof in overall quality evaluation. A test solution and a control mixed solution are detected by using liquid chromatography to obtain achromatogram map and a fingerprint is constructed; and overall quality evaluation isperformed on a preparation for muscle relaxing and blood circulation stimulation by combining principal component analysis (PCA), cluster analysis (CA), and orthogonal partial least squares discriminant analysis (OPLS-DA). The result shows that the established fingerprint combined with the chemical pattern recognition method is stable and simple; four common peaks with the largest differences aredetermined; and thus the fingerprint can be used for evaluating the quality of the preparation for muscle relaxing and blood circulation stimulation.

Description

technical field [0001] The invention belongs to the field of drug quality control, and in particular relates to a fingerprint of a preparation for relaxing tendons and promoting blood circulation and its application in overall quality evaluation. Background technique [0002] It is composed of 10 single herbal medicines: safflower, Cyperus cyperi (made), dog ridge (made), xiangjia bark, mistletoe, eupatorium leaf, genus chinensis, twig vine, gallinaceous vine, and natural copper (forged). The Shujinhuoxue preparation has the effects of promoting blood circulation, dispelling blood stasis, relaxing tendons and activating collaterals, and is mainly used for muscle and bone pain, bruises, rheumatoid arthritis and other diseases. The skin has the effect of dispelling rheumatism and strengthening bones and muscles. It is mainly used for wind-cold-damp arthralgia, soreness of the waist and knees, etc. Clinical data show that the effect is good. According to different dosage forms...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/36
CPCG01N30/02G01N30/06G01N30/36G01N2030/047
Inventor 颜继忠张慧田清华张亚徐聪陈燕
Owner ZHEJIANG UNIV OF TECH
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