Fingerprint of preparation for muscle relaxing and blood circulation stimulation and application thereof in overall quality evaluation

A technology for relaxing tendons and promoting blood circulation and fingerprinting, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problem of ignoring the synergy of multiple mechanisms of the diversity of traditional Chinese medicine components, difficult to comprehensively measure the efficacy and quality of traditional Chinese medicine, and the difficulty of quantitative indicators to meet quality standards. and other problems to achieve the effect of quality control, high column efficiency and pressure reduction

Inactive Publication Date: 2019-11-08
ZHEJIANG UNIV OF TECH
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And there are certain problems in the current ministerial quality standards. At present, there are only thin-layer chromatography to qualitatively identify Cyperus cyperi, safflower, dog ridge and xiangjiapi, and HPLC to determine the content of 4-methoxy salicylaldehyde
However, the formula of this variety is complex and the ingredients are diverse, and a single quanti

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fingerprint of preparation for muscle relaxing and blood circulation stimulation and application thereof in overall quality evaluation
  • Fingerprint of preparation for muscle relaxing and blood circulation stimulation and application thereof in overall quality evaluation
  • Fingerprint of preparation for muscle relaxing and blood circulation stimulation and application thereof in overall quality evaluation

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Example 1

[0037] Optimization of liquid chromatography detection conditions for Shujinhuoxue preparations:

[0038] The present invention is suitable for methanol-water-acetic acid (volume ratio 47:50:3), acetonitrile-0.1vt% phosphoric acid aqueous solution, acetonitrile-0.2vt% phosphoric acid aqueous solution, acetonitrile-0.2vt% formic acid aqueous solution, methanol-0.2vt% formic acid aqueous solution, etc. Different ratios and types of elution systems were screened, and different wavelengths of 264nm, 270nm, 277nm, and 280nm were investigated. When the wavelength was 277nm and the mobile phase was acetonitrile-0.2vt% formic acid aqueous solution, the chromatographic peak resolution was higher. The total number is larger, the peak purity is better, more components can be separated and the content is higher.

[0039] The present invention has different extraction methods for Shujinhuoxue preparations: heating, reflux and ultrasound, screening, and the types of solvents: m...

Example Embodiment

[0040] Example 2

[0041] Construction of a fingerprint of Shujinhuoxue preparation and its application in overall quality evaluation

[0042] Shujinhuoxue preparations are made of 10 medicinal materials such as safflower, Cyperus rotundus, dog ridge, mistletoe, Echinacea, Eupatorium leaf, Xiangjia bark, Caulis spatholobi, Trachelospermi Caulis, and natural copper. Its dosage forms include: Common pharmaceutical dosage forms such as tablets and capsules. According to the requirements of fingerprint spectrum, the peak area of ​​ten common peaks is greater than 90% of the total peak area, and 6 chromatographic peaks are identified by mass spectrometry. Among them, peak 4 is phellodendron and peak 5 is hydroxysafflor yellow A. Peak 6 is phenylhexanoic acid, peak 8 is noroxoside, peak 9 is rosmarinic acid, and peak 10 is 4-methoxysalicylic aldehyde. The standard products available on the market include hydroxysafflor yellow A, rosmarinic acid and 4-methoxysalicylic aldehyde. Therefor...

Example Embodiment

[0079] Example 3

[0080] The chemical pattern recognition analysis of Shujinhuoxue preparation includes the following steps:

[0081] (1) Principal component analysis (PCA)

[0082] PCA analysis was performed on 23 batches of Shujinhuoxue preparations, with 10 common peak areas as variables, using SIMCA 13.0 statistical software to select the top three principal components with the largest contribution rate. The total contribution rate of the first three principal components was 71.08% , That is, the first three main components can reflect the basic characteristics of Shujinhuoxue preparations. Calculate the principal component scores of 23 batches of Shujinhuoxue preparations. The principal component scores of 23 batches of samples are as follows Figure 4 As shown, the load diagram of 10 indicators in 23 batches of samples is as Figure 5 Shown.

[0083] by Figure 4 It can be seen that it can be divided into 3 categories, S1, S2, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12, S13, S1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention, which belongs to the field of drug quality control, discloses a fingerprint of preparation for muscle relaxing and blood circulation stimulation and application thereof in overall quality evaluation. A test solution and a control mixed solution are detected by using liquid chromatography to obtain achromatogram map and a fingerprint is constructed; and overall quality evaluation isperformed on a preparation for muscle relaxing and blood circulation stimulation by combining principal component analysis (PCA), cluster analysis (CA), and orthogonal partial least squares discriminant analysis (OPLS-DA). The result shows that the established fingerprint combined with the chemical pattern recognition method is stable and simple; four common peaks with the largest differences aredetermined; and thus the fingerprint can be used for evaluating the quality of the preparation for muscle relaxing and blood circulation stimulation.

Description

technical field [0001] The invention belongs to the field of drug quality control, and in particular relates to a fingerprint of a preparation for relaxing tendons and promoting blood circulation and its application in overall quality evaluation. Background technique [0002] It is composed of 10 single herbal medicines: safflower, Cyperus cyperi (made), dog ridge (made), xiangjia bark, mistletoe, eupatorium leaf, genus chinensis, twig vine, gallinaceous vine, and natural copper (forged). The Shujinhuoxue preparation has the effects of promoting blood circulation, dispelling blood stasis, relaxing tendons and activating collaterals, and is mainly used for muscle and bone pain, bruises, rheumatoid arthritis and other diseases. The skin has the effect of dispelling rheumatism and strengthening bones and muscles. It is mainly used for wind-cold-damp arthralgia, soreness of the waist and knees, etc. Clinical data show that the effect is good. According to different dosage forms...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02G01N30/06G01N30/36
CPCG01N30/02G01N30/06G01N30/36G01N2030/047
Inventor 颜继忠张慧田清华张亚徐聪陈燕
Owner ZHEJIANG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap