Method for taking DNA as text information efficient storage medium

Abstract

The invention provides a method for taking DNA as an efficient text information storage medium, which is used for storing text information and is beneficial to culture protection. The method for taking DNA as a text information efficient storage medium comprises a storage process and a decoding process. The storage process comprises the following steps: establishing an information sequence, dividing text content into a plurality of storage units, and converting each storage unit into a DNA sequence by utilizing DNA tool software, thereby converting the whole text into a plurality of DNA sequences; adding 20bp protection sequences to the two ends of the information sequence respectively; adding primer binding regions at two ends of the protection sequence. The decoding process comprises thefollowing steps: culturing bacteria, extracting plasmids, carrying out polymerase chain reaction to obtain an amplification product, carrying out agarose gel electrophoresis and imaging identification on the amplification product, sequencing the amplification product by using a Sanger sequencing method, and reducing the DNA sequence obtained by sequencing into a Chinese character text by using DNATools software.

Description

technical field [0001] The invention relates to the technical field of DNA storage, in particular to a method for using DNA as an efficient storage medium for text information. Background technique [0002] The 21st century is an era of rapid development of information technology, and the amount of information is increasing exponentially. The long-term stable storage of this information has become the focus of attention. The current devices used for data storage such as paper, magnetic media and silicon chips are not ideal storage devices. First of all, the data storage density of silicon chips is limited, and the storage quality of tapes will decline rapidly as the number of times of use increases. The cost of silicon chips is high in the manufacturing process, and a large amount of harmful chemicals will be used, such as silicon tetrachloride, cyanide, dichloroethane, etc., and a large amount of water resources and fossil fuels will be consumed. According to statistics, ab...

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Problems solved by technology

The cost of silicon chips is high in the manufacturing process, and a large amount of harmful chemicals will be used, such as silicon tetrachloride, cyanide, dichloroethane, etc., and a large amount of water resources and fossil fuels will be consumed. According to statistics, about one-third 1 in 2 workers suffer from diseases caused by silicon pollution

Secondly, the larger the capacity of storing information, the larger the volume of the storage device, and there will be a lot of inconvenience in carrying, storing and maintaining the device. Moreover, discarded devices are not easy to degrade and easily cause environmental pollution. Silicon is also a non-renewable resource. a day will be exhausted

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