Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of extraction method of tea saponin

An extraction method and technology of tea saponin, applied in chemical instruments and methods, steroids, organic chemistry, etc., can solve the problems of limiting the application range of tea saponin, low yield and purity of tea saponin, and high cost of methanol. Achieve the effect of improving practical application value, improving safety issues, and improving extraction rate

Active Publication Date: 2020-09-18
HEFEI UNIV
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of the water immersion method is that the yield and purity of tea saponin are low, which limits the application range of tea saponin
The organic solvent method usually uses aqueous methanol or aqueous ethanol as the extraction agent. Due to the high cost and toxicity of methanol, ethanol is mostly used for extraction. However, the advantage of using ethanol is that the method takes a short time, but its purity is not high enough. needs to be further improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of extraction method of tea saponin that the present invention proposes, comprises the following steps:

[0023] S1. Pulverize the tea meal, sieve it, add petroleum ether to it, the ratio of material to liquid is 1:3, stir for 20 minutes, and centrifuge to obtain defatted tea meal;

[0024] S2. Infuse water vapor into the defatted tea dregs to keep the internal gas humidity at 20%, keep it sealed for 20 minutes, then take out the wet tea dregs, freeze at -12°C for 22 hours, then thaw at room temperature, and repeat freezing- The thawing operation is performed once to obtain the pretreated tea meal;

[0025] S3, place the pretreated tea dregs in the percolation cylinder, add 70% ethanol solution and soak for 30min, then use 70% ethanol solution to carry out percolation treatment, the total amount of ethanol solution for percolation is 4 times of the initial weight of tea dregs, The percolation speed is 3mL of percolation liquid per minute per 1000g of initial tea...

Embodiment 2

[0029] A kind of extraction method of tea saponin that the present invention proposes, comprises the following steps:

[0030] S1. Pulverize the tea meal, sieve it, add petroleum ether to it, the ratio of material to liquid is 1:5, stir for 30 minutes, and centrifuge to obtain defatted tea meal;

[0031] S2. Pass water vapor into the defatted tea leaves, keep the internal gas humidity at 25%, keep it sealed for 40 minutes, then take out the wet tea leaves, freeze them at -18°C for 26 hours, then thaw them at room temperature, and repeat freezing- The thawing operation was performed 3 times to obtain the pretreated tea meal;

[0032] S3, place the pretreated tea dregs in the percolation cylinder, add 80% ethanol solution and soak for 40min, then use 80% ethanol solution to carry out percolation treatment, the total amount of ethanol solution for percolation is 5 times of the initial weight of tea dregs, The percolation speed is 5mL of percolation liquid per 1000g of initial te...

Embodiment 3

[0036] A kind of extraction method of tea saponin that the present invention proposes, comprises the following steps:

[0037] S1. Pulverize the tea meal, sieve it, add petroleum ether to it, the ratio of material to liquid is 1:3.5, stir for 20 minutes, and centrifuge to obtain defatted tea meal;

[0038] S2. Infuse water vapor into the defatted tea leaves to keep the internal gas humidity at 22%, keep it sealed for 25 minutes, then take out the wet tea leaves, freeze at -14°C for 23 hours, then thaw at room temperature, and repeat freezing- The thawing operation is performed once to obtain the pretreated tea meal;

[0039] S3, the pretreated tea dregs is placed in the percolation cylinder, add 75% ethanol solution and soak for 35min, then adopt 75% ethanol solution to carry out percolation treatment, the total amount of ethanol solution for percolation is 4 times of the initial weight of tea dregs, The percolation speed is 3.5mL of percolation liquid per minute per 1000g of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an extraction method of tea saponin, and relates to the technical field of tea saponin extraction. The extraction method comprises the following steps: 1) crushing and sievingtea seed meal, adding petroleum ether into the tea seed meal, stirring the mixture, and carrying out centrifugal separation to obtain degreased tea seed meal; 2) feeding water vapor into the degreasedtea seed meal, sealing the degreased tea seed meal, removing the wet tea seed meal, freezing and then unfreezing the tea seed meal to obtain pretreated tea seed meal; 3) adding the pretreated tea seed meal into a percolation cylinder, adding a 70-80% ethanol solution for soaking, then performing percolation, and collecting a percolate; 4) recovering ethanol from the percolate under reduced pressure, adding water, adjusting the pH value to 9.0-10.0, and standing and filtering the liquid to obtain a refined extract; 5) dropwise adding a hydrochloric acid solution into the refined extracting solution until the pH value is 2-3, and filtering, washing the liquid and then dissolving the liquid with a 95% ethanol solution, dropwise adding acetone, and carrying out standing, filtering and dryingtreatment. The extraction method is simple to operate, and the obtained tea saponin is light in color, high in extraction rate and high in purity, so that the practical application value of the tea saponin is improved.

Description

technical field [0001] The invention relates to the technical field of tea saponin extraction, in particular to a method for extracting tea saponin. Background technique [0002] Tea saponin, also known as tea saponin, is a kind of glycoside compound extracted from tea tree seeds. It is a kind of natural surfactant with good performance. It can be widely used in light industry, chemical industry, pesticide, feed, breeding, textile , oil extraction, mining, building materials and highway construction and other fields, manufacturing emulsifiers, detergents, pesticide auxiliaries, feed additives, crab and shrimp breeding protection agents, textile auxiliaries, oilfield foam agents, mining flotation agents and aerated concrete Foam stabilizer and concrete admixture - antifreeze, etc. [0003] At present, the methods of extracting tea saponin at home and abroad mainly include water immersion method and organic solvent method, as well as new methods popularized on this basis. Th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08
CPCC07H1/08C07H15/256C07J63/008
Inventor 朱仁发
Owner HEFEI UNIV