A method for assessing sarcoplasmic reticulum calcium leakage

Abstract

The present invention relates to a method for evaluating sarcoplasmic reticulum calcium leakage. The method comprises the following steps of : step 1) carrying out an isolated separation and culture experiment of myocardial cells and recording calcium activity of the myocardial cells under a laser confocal microscope; step 2) standardizing a fluorescent image collected in the step 1), and then performing fluorescent intensity statistics on the standardized processed image to obtain an amplitude distribution histogram; step 3) performing left-right asymmetric Gaussian fitting with a peak valueof a curve surround by the amplitude distribution histogram in the step 2) as the center, and obtaining fluorescence deviation parameters by calculating the area on both sides of the center of the original curve and the fitting curve; and step 4) carrying out time difference on the original image to obtain a flow chart, repeating the operation of the step 3) on the flow chart to obtain an amplitude distribution histogram, performing asymmetric Gaussian fitting, and obtaining flow deviation parameters through calculation to obtain two new parameters describing the sarcoplasmic reticulum calciumleakage. The method for evaluating sarcoplasmic reticulum calcium leakage can more quickly, effectively and accurately evaluate the calcium leakage level of the sarcoplasmic reticulum.

Description

technical field [0001] The invention belongs to the technical field of biomedical image processing, and in particular relates to a method for evaluating sarcoplasmic reticulum calcium leakage. Background technique [0002] The number of patients with cardiovascular disease is increasing day by day, and it has become the main cause of death among Chinese residents. Calcium signal transmission in which calcium ions participate is an important mechanism that affects the physiological function of the heart. Whether it is normal or not directly determines and regulates the excitation and contraction of cardiomyocytes. Studies have shown that the occurrence of cardiovascular diseases such as arrhythmia and heart failure is closely related to the abnormal calcium signal of cardiomyocytes. Therefore, the concentration of calcium ions in the cytoplasm is the cornerstone of the subsequent regulation of cardiomyocyte activity by calcium ions. [0003] There are many forms of calcium l...

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Problems solved by technology

However, the measurement of calcium leakage by the above process is indirect, and the myocardial cells will also produce unpredictable changes under abnormal physiological conditions, so the pathophysiological value of calcium leakage measured at this time will be limited

In addition, in terms of intuitive measurement, since the increase of calcium load in the sarcoplasmic reticulum will increase the frequency of calcium sparks exponentially, although calcium sparks only represent a part of calcium leakage, they are still an effective indicator for measuring sarcoplasmic reticulum calcium leakage

However, due to the existence of empirical parameters in the existing calcium spark detection methods, the results are largely affected by the subjective factors of researchers; and the amplitude and duration of some calcium sparks vary greatly, which also makes the detection more difficult and difficult to control its error from the true value

Calcium leakage from calcium spark measurements is therefore not as expected

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