Method of evaluating edible safety of transgenic plants

Abstract

The invention discloses a method of evaluating the edible safety of transgenic plants. In the method, a transgenic plant, a plant A, a plant B, and a plant C are used to feed an animal, the plant A isa non-transgenic parent of the transgenic plant, the plant B is a different plant belonging to the same cultivar as the plant A, and the plant C is a different plant belonging to a different cultivarfrom the plant A; the intestinal metabolome components in the cecum contents of the animal are detected, the following judgments are made: if the differences between the intestinal metabolome components with the transgenic plant fed and the intestinal metabolome components with the plant A fed are lower than the differences between the intestinal metabolome components with the plant A fed and theintestinal metabolome components with the plant B fed and / or the differences between the intestinal metabolome components with the plant A fed and the intestinal metabolome components with the plantC fed, the transgenic plant has edible safety. The method provided by the invention can evaluate the edible safety of a transgenic plant and has important application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for evaluating the edible safety of transgenic plants. Background technique [0002] Genetically modified food refers to food processed and produced with genetically modified organisms as raw materials. According to the different sources of genetically modified food, genetically modified food is generally divided into genetically modified plant food and genetically modified animal food. milk). With the increase of genetically modified animals and plants and the improvement of commercial application, the safety of genetically modified animals, plants and their food has become the focus of social attention. [0003] One of the important methods for evaluating the food safety of genetically modified foods is to study the "substantial equivalence" of the effects of genetically modified foods on animal models. Intestinal metabolome refers to the collection of low-m...

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Problems solved by technology

[0004] In traditional detection methods, feces are often used as the sample source of intestinal metabolome components, which has the following limitations: on the one hand, the digestion and absorption of food components and the interaction with intestinal cells are mainly completed in the small intestine, and feces and Metabolites in the intestinal tract vary greatly, which affects the accuracy of judgment; on the other hand, feces contain a large amount of digestive juice in the large intestine, and the internal environment of the large intestine is different from that of the small intestine, which will also lead to greater production of unstable substances such as bile acids. changes, which in turn affect the reliability of the judgment

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