A method for evaluating the food safety of transgenic plants

Abstract

The invention discloses a method for evaluating the edible safety of transgenic plants. The method is to feed animals with transgenic plants, plant A, plant B and plant C respectively, plant A is the non-transgenic parent of the transgenic plant, plant B is a different plant belonging to the same cultivar as plant A, and plant C is the same as plant A. Different plants belonging to different cultivars; detect the intestinal metabolome components in the cecal contents of animals, and make the following judgments: If "the intestinal metabolome components of fed transgenic plants and the intestinal metabolome components of fed plant The difference between the gut metabolome components fed plant A and the gut metabolome fraction fed plant B” was lower than the difference between the gut metabolome components fed plant A and / or the gut metabolome components fed plant A If the difference between the gut metabolome composition of the feeder and the fed plant C", the transgenic plant is safe to eat. The method provided by the invention can evaluate the food safety of transgenic plants, and has important application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for evaluating the edible safety of transgenic plants. Background technique [0002] Genetically modified food refers to food processed and produced with genetically modified organisms as raw materials. According to the different sources of genetically modified food, genetically modified food is generally divided into genetically modified plant food and genetically modified animal food. milk). With the increase of genetically modified animals and plants and the improvement of commercial application, the safety of genetically modified animals, plants and their food has become the focus of social attention. [0003] One of the important methods for evaluating the food safety of genetically modified foods is to study the "substantial equivalence" of the effects of genetically modified foods on animal models. Intestinal metabolome refers to the collection of low-m...

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Problems solved by technology

[0004] In traditional detection methods, feces are often used as the sample source of intestinal metabolome components, which has the following limitations: on the one hand, the digestion and absorption of food components and the interaction with intestinal cells are mainly completed in the small intestine, and feces and Metabolites in the intestinal tract vary greatly, which affects the accuracy of judgment; on the other hand, feces contain a large amount of digestive juice in the large intestine, and the internal environment of the large intestine is different from that of the small intestine, which will also lead to greater production of unstable substances such as bile acids. changes, which in turn affect the reliability of the judgment

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